The Fission Yeast dis3+ Gene Encodes a 110-kDa Essential Protein Implicated in Mitotic Control

Noriyuki Kinoshita, Mark Goebl, Mitsuhiro Yanagida

Research output: Contribution to journalArticle

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Abstract

The fission yeast mutant dis3-54 is defective in mitosis and fails in chromosome disjunction. Its phenotype is similar to that of dis2-11, a mutant with a mutation in the type 1 protein phosphatase gene. We cloned the dis3+ gene by transformation. Nucleotide sequencing predicts a coding region of 970 amino acids interrupted by a 164-bp intron at the 65th codon. The predicted dis3+ protein shares a weak but significant similarity with the budding yeast SSD1 or SRK1 gene product, the gene for which is a suppressor for the absence of a protein phosphatase SIT4 gene or the BCY1 regulatory subunit of cyclic AMP-dependent protein kinase. Anti-dis3 antibodies recognized the 110-kDa dis3+ gene product, which is part of a 250- to 350-kDa oligomer and is enriched in the nucleus. The cellular localization of the dis3+ protein is reminiscent of that of the dis2+ protein, but these two proteins do not form a complex. A type 1 protein phosphatase activity in the dis3-54 mutant extracts is apparently not affected. The dis3+ gene is essential for growth; gene disruptant cells do not germinate and fail in cell division. Increased dis3+ gene dosage reverses the Ts+ phenotype of a cdc25 wee1 strain, as does increased type 1 protein phosphatase gene dosage. Double mutant dis3 dis2 is lethal even at the permissive temperature, suggesting that the dis2+ and dis3+ genes may be functionally overlapped. The role of the dis3+ gene product in mitosis is unknown, but this gene product may be directly or indirectly involved in the regulation of mitosis.

Original languageEnglish
Pages (from-to)5839-5847
Number of pages9
JournalMolecular and Cellular Biology
Volume11
Issue number12
StatePublished - Dec 1991

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Schizosaccharomyces
Genes
Proteins
Protein Phosphatase 1
Mitosis
Gene Dosage
Phenotype
Saccharomycetales
Essential Genes
Cyclic AMP-Dependent Protein Kinases
Codon
Cell Division
Introns
Anti-Idiotypic Antibodies
Nucleotides
Chromosomes
Amino Acids
Mutation
Temperature

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

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The Fission Yeast dis3+ Gene Encodes a 110-kDa Essential Protein Implicated in Mitotic Control. / Kinoshita, Noriyuki; Goebl, Mark; Yanagida, Mitsuhiro.

In: Molecular and Cellular Biology, Vol. 11, No. 12, 12.1991, p. 5839-5847.

Research output: Contribution to journalArticle

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