The Giant Danio (D. Aequipinnatus) as A Model of Cardiac Remodeling and Regeneration

Pascal J. Lafontant, Alan R. Burns, Jamie A. Grivas, Mary A. Lesch, Tanmoy D. Lala, Sean P. Reuter, Loren Field, Tyler D. Frounfelter

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The paucity of mammalian adult cardiac myocytes (CM) proliferation following myocardial infarction (MI) and the remodeling of the necrotic tissue that ensues, result in non-regenerative repair. In contrast, zebrafish (ZF) can regenerate after an apical resection or cryoinjury of the heart. There is considerable interest in models where regeneration proceeds in the presence of necrotic tissue. We have developed and characterized a cautery injury model in the giant danio (GD), a species closely related to ZF, where necrotic tissue remains part of the ventricle, yet regeneration occurs. By light and transmission electron microscopy (TEM), we have documented four temporally overlapping processes: (1) a robust inflammatory response analogous to that observed in MI, (2) concomitant proliferation of epicardial cells leading to wound closure, (3) resorption of necrotic tissue and its replacement by granulation tissue, and (4) regeneration of the myocardial tissue driven by 5-EDU and [ 3H]thymidine incorporating CMs. In conclusion, our data suggest that the GD possesses robust repair mechanisms in the ventricle and can serve as an important model of cardiac inflammation, remodeling and regeneration.

Original languageEnglish
Pages (from-to)234-248
Number of pages15
JournalAnatomical Record
Volume295
Issue number2
DOIs
StatePublished - Feb 2012

Fingerprint

Danio
Regeneration
regeneration
myocardial infarction
Zebrafish
Danio rerio
inflammation
repair
Myocardial Infarction
Cautery
granulation tissue
Granulation Tissue
Wounds and Injuries
resorption
resection
thymidine
Transmission Electron Microscopy
Cardiac Myocytes
animal injuries
Thymidine

Keywords

  • Cardiomyocytes
  • Giant danio
  • Heart
  • Inflammation
  • Regeneration
  • Remodeling
  • Zebrafish

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Ecology, Evolution, Behavior and Systematics
  • Biotechnology

Cite this

Lafontant, P. J., Burns, A. R., Grivas, J. A., Lesch, M. A., Lala, T. D., Reuter, S. P., ... Frounfelter, T. D. (2012). The Giant Danio (D. Aequipinnatus) as A Model of Cardiac Remodeling and Regeneration. Anatomical Record, 295(2), 234-248. https://doi.org/10.1002/ar.21492

The Giant Danio (D. Aequipinnatus) as A Model of Cardiac Remodeling and Regeneration. / Lafontant, Pascal J.; Burns, Alan R.; Grivas, Jamie A.; Lesch, Mary A.; Lala, Tanmoy D.; Reuter, Sean P.; Field, Loren; Frounfelter, Tyler D.

In: Anatomical Record, Vol. 295, No. 2, 02.2012, p. 234-248.

Research output: Contribution to journalArticle

Lafontant, PJ, Burns, AR, Grivas, JA, Lesch, MA, Lala, TD, Reuter, SP, Field, L & Frounfelter, TD 2012, 'The Giant Danio (D. Aequipinnatus) as A Model of Cardiac Remodeling and Regeneration', Anatomical Record, vol. 295, no. 2, pp. 234-248. https://doi.org/10.1002/ar.21492
Lafontant PJ, Burns AR, Grivas JA, Lesch MA, Lala TD, Reuter SP et al. The Giant Danio (D. Aequipinnatus) as A Model of Cardiac Remodeling and Regeneration. Anatomical Record. 2012 Feb;295(2):234-248. https://doi.org/10.1002/ar.21492
Lafontant, Pascal J. ; Burns, Alan R. ; Grivas, Jamie A. ; Lesch, Mary A. ; Lala, Tanmoy D. ; Reuter, Sean P. ; Field, Loren ; Frounfelter, Tyler D. / The Giant Danio (D. Aequipinnatus) as A Model of Cardiac Remodeling and Regeneration. In: Anatomical Record. 2012 ; Vol. 295, No. 2. pp. 234-248.
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