The H8 antigen of pathogenic Neisseriae

J. P. Woods, E. L. Aho, D. S. Barritt, J. R. Black, T. D. Connell, T. H. Kawula, Stanley Spinola, J. G. Cannon

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We cloned and sequenced the H8 gene from N. meningitidis FAM18. The predicted amino acid sequence included a consensus lipoprotein signal sequence processing site, consistent with lipid modification that could account for the unusual electrophoretic and solubilization properties of H8. The amino acid sequence was rich in alanine and proline, especially in an imperfectly periodic region near the amino terminus, which encompassed the epitope recognized by available monoclonal antibodies. In a panel of neisserial strains, the presence of DNA homologous to the H8 gene correlated with the expression of an H8 protein. We cloned a gene from N. meningitidis JB515 that was distinct from the H8 gene but encoded a protein also recognized by an anti-H8 monoclonal antibody. Mice were not protected from meningococcemia by passive immunization with such an antibody.

Original languageEnglish (US)
Pages (from-to)533-536
Number of pages4
JournalAntonie van Leeuwenhoek
Volume53
Issue number6
DOIs
StatePublished - Nov 1987
Externally publishedYes

Fingerprint

Neisseria
Antigens
Genes
Amino Acid Sequence
Monoclonal Antibodies
Passive Immunization
Protein Sorting Signals
Proline
Alanine
Lipoproteins
Epitopes
Proteins
Lipids
Antibodies
DNA

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Woods, J. P., Aho, E. L., Barritt, D. S., Black, J. R., Connell, T. D., Kawula, T. H., ... Cannon, J. G. (1987). The H8 antigen of pathogenic Neisseriae. Antonie van Leeuwenhoek, 53(6), 533-536. https://doi.org/10.1007/BF00415513

The H8 antigen of pathogenic Neisseriae. / Woods, J. P.; Aho, E. L.; Barritt, D. S.; Black, J. R.; Connell, T. D.; Kawula, T. H.; Spinola, Stanley; Cannon, J. G.

In: Antonie van Leeuwenhoek, Vol. 53, No. 6, 11.1987, p. 533-536.

Research output: Contribution to journalArticle

Woods, JP, Aho, EL, Barritt, DS, Black, JR, Connell, TD, Kawula, TH, Spinola, S & Cannon, JG 1987, 'The H8 antigen of pathogenic Neisseriae', Antonie van Leeuwenhoek, vol. 53, no. 6, pp. 533-536. https://doi.org/10.1007/BF00415513
Woods JP, Aho EL, Barritt DS, Black JR, Connell TD, Kawula TH et al. The H8 antigen of pathogenic Neisseriae. Antonie van Leeuwenhoek. 1987 Nov;53(6):533-536. https://doi.org/10.1007/BF00415513
Woods, J. P. ; Aho, E. L. ; Barritt, D. S. ; Black, J. R. ; Connell, T. D. ; Kawula, T. H. ; Spinola, Stanley ; Cannon, J. G. / The H8 antigen of pathogenic Neisseriae. In: Antonie van Leeuwenhoek. 1987 ; Vol. 53, No. 6. pp. 533-536.
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