The H8 antigen of pathogenic Neisseriae

J. P. Woods, E. L. Aho, D. S. Barritt, J. R. Black, T. D. Connell, T. H. Kawula, S. M. Spinola, J. G. Cannon

Research output: Contribution to journalArticle

3 Scopus citations


We cloned and sequenced the H8 gene from N. meningitidis FAM18. The predicted amino acid sequence included a consensus lipoprotein signal sequence processing site, consistent with lipid modification that could account for the unusual electrophoretic and solubilization properties of H8. The amino acid sequence was rich in alanine and proline, especially in an imperfectly periodic region near the amino terminus, which encompassed the epitope recognized by available monoclonal antibodies. In a panel of neisserial strains, the presence of DNA homologous to the H8 gene correlated with the expression of an H8 protein. We cloned a gene from N. meningitidis JB515 that was distinct from the H8 gene but encoded a protein also recognized by an anti-H8 monoclonal antibody. Mice were not protected from meningococcemia by passive immunization with such an antibody.

Original languageEnglish (US)
Pages (from-to)533-536
Number of pages4
JournalAntonie van Leeuwenhoek
Issue number6
StatePublished - Nov 1 1987
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Fingerprint Dive into the research topics of 'The H8 antigen of pathogenic Neisseriae'. Together they form a unique fingerprint.

  • Cite this

    Woods, J. P., Aho, E. L., Barritt, D. S., Black, J. R., Connell, T. D., Kawula, T. H., Spinola, S. M., & Cannon, J. G. (1987). The H8 antigen of pathogenic Neisseriae. Antonie van Leeuwenhoek, 53(6), 533-536.