The heat-shock response in cultured cells exposed to ethanol and its metabolites

K. H. Walsh, David Crabb

Research output: Contribution to journalArticle

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Abstract

Many stress, including elevated temperature and exposure to toxins or heavy metals, activate a stereotyped response of cultured cells known as the heat-shock response. The products of several highly conserved heat-shock genes (heat-shock proteins) protect the cells against subsequent stresses. The intra-cellular signal for the response is unknown, but may include the presence of damaged and abnormal proteins in the cell. Ethanol at high concentration (1.3 mol/L) has been shown to activate the heat-shock response in hamster ovary fibroblasts, suggesting that this response might be an important consequence of exposure of cells to ethanol and might mediate some of its cellular toxicity. To determine if lower concentrations of ethanol or its metabolites could activate a heat-shock response, we transfected COS-1 cells with a reported gene (the Drosophila 70 kd heat-shock protein promoter fused to the β-galactosidase gene), then exposed them to various compounds. Exposure to heat induced at least a threefold to fourfold increase in β-galactosidase activity, whereas 1.3 mol/L ethanol induced a sixfold increase. Lower concentrations of ethanol (100 to 500 mmol/L) or acetaldehyde (100 to 500 μmol/L) did not induce a measurable heat-shock response. Similarly, high concentrations of metabolites generated during ethanol oxidation (10 mmol/L lactate or acetate) did not induce the response. We conclude that the heat-shock response cannot be detected with this assay system in COS-1 cells after short exposure to physiologically achievable concentrations of ethanol or its metabolites. However, it is possible that it is induced at a low level or in tissues directly exposed to alcoholic beverages 9e.g., oropharynx, esophagus, and stomach).

Original languageEnglish
Pages (from-to)563-567
Number of pages5
JournalJournal of Laboratory and Clinical Medicine
Volume114
Issue number5
StatePublished - 1989

Fingerprint

Heat-Shock Response
Metabolites
Cultured Cells
Ethanol
Cells
Galactosidases
Genes
COS Cells
Heat-Shock Proteins
Hot Temperature
Alcoholic Beverages
Oropharynx
HSP70 Heat-Shock Proteins
Acetaldehyde
Fibroblasts
Heavy Metals
Cricetinae
Esophagus
Drosophila
Toxicity

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

The heat-shock response in cultured cells exposed to ethanol and its metabolites. / Walsh, K. H.; Crabb, David.

In: Journal of Laboratory and Clinical Medicine, Vol. 114, No. 5, 1989, p. 563-567.

Research output: Contribution to journalArticle

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