The helix-loop-helix protein Id2 is overexpressed in human pancreatic cancer

Jörg Kleeff, Toshiyuki Ishiwata, Helmut Friess, Markus W. Büchler, Mark A. Israel, Murray Korc

Research output: Contribution to journalArticlepeer-review

94 Scopus citations

Abstract

Id2 belongs to the Id family of helix-loop-helix (HLH) proteins, which upon heterodimerization with basic HLH proteins prevent basic HLH proteins from DNA binding. Proteins of the Id family act as negative regulatory transcriptional factors, and their expression correlates with cell proliferation and arrested differentiation in many celt lineages. In this study, we characterized the expression of Id2 in normal and cancerous pancreatic tissues. Pancreatic cancers markedly overexpressed Id2 mRNA in comparison to the normal pancreas. Furthermore, there was abundant Id2 immunoreactivity in the cancer cells within the pancreatic tumor mass. In PANC-1 human pancreatic cancer cells, steady-state Id2 mRNA levels increased upon serum addition and decreased after induction of differentiation with either sodium butyrate or 12-O-tetradecanoylphorbol-13-acetate. Inhibition of Id2 expression with Id2 antisense oligonucleotides inhibited the growth of these cells, whereas random and sense oligonucleotides were without effect. These findings suggest that Id2 may have a role in human pancreatic cancer.

Original languageEnglish (US)
Pages (from-to)3769-3772
Number of pages4
JournalCancer Research
Volume58
Issue number17
StatePublished - Sep 1 1998

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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