The helix-loop-helix protein Id2 is overexpressed in human pancreatic cancer

Jörg Kleeff, Toshiyuki Ishiwata, Helmut Friess, Markus W. Büchler, Mark A. Israel, Murray Korc

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Id2 belongs to the Id family of helix-loop-helix (HLH) proteins, which upon heterodimerization with basic HLH proteins prevent basic HLH proteins from DNA binding. Proteins of the Id family act as negative regulatory transcriptional factors, and their expression correlates with cell proliferation and arrested differentiation in many celt lineages. In this study, we characterized the expression of Id2 in normal and cancerous pancreatic tissues. Pancreatic cancers markedly overexpressed Id2 mRNA in comparison to the normal pancreas. Furthermore, there was abundant Id2 immunoreactivity in the cancer cells within the pancreatic tumor mass. In PANC-1 human pancreatic cancer cells, steady-state Id2 mRNA levels increased upon serum addition and decreased after induction of differentiation with either sodium butyrate or 12-O-tetradecanoylphorbol-13-acetate. Inhibition of Id2 expression with Id2 antisense oligonucleotides inhibited the growth of these cells, whereas random and sense oligonucleotides were without effect. These findings suggest that Id2 may have a role in human pancreatic cancer.

Original languageEnglish (US)
Pages (from-to)3769-3772
Number of pages4
JournalCancer Research
Volume58
Issue number17
StatePublished - Sep 1 1998
Externally publishedYes

Fingerprint

Pancreatic Neoplasms
Messenger RNA
Proteins
Butyric Acid
Antisense Oligonucleotides
DNA-Binding Proteins
Tetradecanoylphorbol Acetate
Oligonucleotides
Pancreas
Neoplasms
Cell Proliferation
Growth
Serum

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Kleeff, J., Ishiwata, T., Friess, H., Büchler, M. W., Israel, M. A., & Korc, M. (1998). The helix-loop-helix protein Id2 is overexpressed in human pancreatic cancer. Cancer Research, 58(17), 3769-3772.

The helix-loop-helix protein Id2 is overexpressed in human pancreatic cancer. / Kleeff, Jörg; Ishiwata, Toshiyuki; Friess, Helmut; Büchler, Markus W.; Israel, Mark A.; Korc, Murray.

In: Cancer Research, Vol. 58, No. 17, 01.09.1998, p. 3769-3772.

Research output: Contribution to journalArticle

Kleeff, J, Ishiwata, T, Friess, H, Büchler, MW, Israel, MA & Korc, M 1998, 'The helix-loop-helix protein Id2 is overexpressed in human pancreatic cancer', Cancer Research, vol. 58, no. 17, pp. 3769-3772.
Kleeff J, Ishiwata T, Friess H, Büchler MW, Israel MA, Korc M. The helix-loop-helix protein Id2 is overexpressed in human pancreatic cancer. Cancer Research. 1998 Sep 1;58(17):3769-3772.
Kleeff, Jörg ; Ishiwata, Toshiyuki ; Friess, Helmut ; Büchler, Markus W. ; Israel, Mark A. ; Korc, Murray. / The helix-loop-helix protein Id2 is overexpressed in human pancreatic cancer. In: Cancer Research. 1998 ; Vol. 58, No. 17. pp. 3769-3772.
@article{4aa9023eacc8498f9b4653878ea9de61,
title = "The helix-loop-helix protein Id2 is overexpressed in human pancreatic cancer",
abstract = "Id2 belongs to the Id family of helix-loop-helix (HLH) proteins, which upon heterodimerization with basic HLH proteins prevent basic HLH proteins from DNA binding. Proteins of the Id family act as negative regulatory transcriptional factors, and their expression correlates with cell proliferation and arrested differentiation in many celt lineages. In this study, we characterized the expression of Id2 in normal and cancerous pancreatic tissues. Pancreatic cancers markedly overexpressed Id2 mRNA in comparison to the normal pancreas. Furthermore, there was abundant Id2 immunoreactivity in the cancer cells within the pancreatic tumor mass. In PANC-1 human pancreatic cancer cells, steady-state Id2 mRNA levels increased upon serum addition and decreased after induction of differentiation with either sodium butyrate or 12-O-tetradecanoylphorbol-13-acetate. Inhibition of Id2 expression with Id2 antisense oligonucleotides inhibited the growth of these cells, whereas random and sense oligonucleotides were without effect. These findings suggest that Id2 may have a role in human pancreatic cancer.",
author = "J{\"o}rg Kleeff and Toshiyuki Ishiwata and Helmut Friess and B{\"u}chler, {Markus W.} and Israel, {Mark A.} and Murray Korc",
year = "1998",
month = "9",
day = "1",
language = "English (US)",
volume = "58",
pages = "3769--3772",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "17",

}

TY - JOUR

T1 - The helix-loop-helix protein Id2 is overexpressed in human pancreatic cancer

AU - Kleeff, Jörg

AU - Ishiwata, Toshiyuki

AU - Friess, Helmut

AU - Büchler, Markus W.

AU - Israel, Mark A.

AU - Korc, Murray

PY - 1998/9/1

Y1 - 1998/9/1

N2 - Id2 belongs to the Id family of helix-loop-helix (HLH) proteins, which upon heterodimerization with basic HLH proteins prevent basic HLH proteins from DNA binding. Proteins of the Id family act as negative regulatory transcriptional factors, and their expression correlates with cell proliferation and arrested differentiation in many celt lineages. In this study, we characterized the expression of Id2 in normal and cancerous pancreatic tissues. Pancreatic cancers markedly overexpressed Id2 mRNA in comparison to the normal pancreas. Furthermore, there was abundant Id2 immunoreactivity in the cancer cells within the pancreatic tumor mass. In PANC-1 human pancreatic cancer cells, steady-state Id2 mRNA levels increased upon serum addition and decreased after induction of differentiation with either sodium butyrate or 12-O-tetradecanoylphorbol-13-acetate. Inhibition of Id2 expression with Id2 antisense oligonucleotides inhibited the growth of these cells, whereas random and sense oligonucleotides were without effect. These findings suggest that Id2 may have a role in human pancreatic cancer.

AB - Id2 belongs to the Id family of helix-loop-helix (HLH) proteins, which upon heterodimerization with basic HLH proteins prevent basic HLH proteins from DNA binding. Proteins of the Id family act as negative regulatory transcriptional factors, and their expression correlates with cell proliferation and arrested differentiation in many celt lineages. In this study, we characterized the expression of Id2 in normal and cancerous pancreatic tissues. Pancreatic cancers markedly overexpressed Id2 mRNA in comparison to the normal pancreas. Furthermore, there was abundant Id2 immunoreactivity in the cancer cells within the pancreatic tumor mass. In PANC-1 human pancreatic cancer cells, steady-state Id2 mRNA levels increased upon serum addition and decreased after induction of differentiation with either sodium butyrate or 12-O-tetradecanoylphorbol-13-acetate. Inhibition of Id2 expression with Id2 antisense oligonucleotides inhibited the growth of these cells, whereas random and sense oligonucleotides were without effect. These findings suggest that Id2 may have a role in human pancreatic cancer.

UR - http://www.scopus.com/inward/record.url?scp=0032170565&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032170565&partnerID=8YFLogxK

M3 - Article

C2 - 9731481

AN - SCOPUS:0032170565

VL - 58

SP - 3769

EP - 3772

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 17

ER -