The highest concentration of primitive hematopoietic progenitor cells in cord blood is found in extremely premature infants

Laura Haneline, Kimberly P. Marshall, D. Clapp

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We used two independent in vitro assays to measure the frequency and proliferative potential of primitive hematopoietic progenitors from the cord blood of 23-41 wk of gestation newborns and adult bone marrow. The frequency of primitive progenitors in the circulating blood cells of infants at 23-31 wk of gestation was significantly greater than the frequency in adult bone marrow or cord blood of more mature newborns. In addition, on a cell to cell basis, the proliferative potential of the primitive progenitors from immature infants (23-31 wk) was greater than in adult bone marrow or cord blood of term newborns. Circulating cord blood cells from immature infants were used as targets for transduction with recombinant retrovirus vectors, and a high efficiency of gene transfer was observed in both primitive and committed progenitors. These data demonstrate that there are major ontogenic shifts in primitive progenitor/stem cell populations in the circulation throughout development as well as programmatic changes in hematopoietic progenitor cell proliferation. In addition, fetal cord blood cells may prove useful targets for genetic manipulation and autologous transplantation.

Original languageEnglish
Pages (from-to)820-825
Number of pages6
JournalPediatric Research
Volume39
Issue number5
StatePublished - May 1996

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Extremely Premature Infants
Hematopoietic Stem Cells
Fetal Blood
Blood Cells
Bone Marrow
Newborn Infant
Stem Cells
Pregnancy
Autologous Transplantation
Retroviridae
Cell Proliferation
Population
Genes

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

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The highest concentration of primitive hematopoietic progenitor cells in cord blood is found in extremely premature infants. / Haneline, Laura; Marshall, Kimberly P.; Clapp, D.

In: Pediatric Research, Vol. 39, No. 5, 05.1996, p. 820-825.

Research output: Contribution to journalArticle

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