The immune response to Haemophilus ducreyi resembles a delayed-type hypersensitivity reaction throughout experimental infection of human subjects

Katherine L. Palmer, Carol T. Schnizlein-Bick, Attilio Orazi, Karla John, Cheng Yen Chen, Antoinette F. Hood, Stanley Spinola

Research output: Contribution to journalArticle

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Abstract

Previous work in 3 subjects infected for 2 weeks indicated that experimental infection with Haemophilus ducreyi recruits CD4 cells to the skin at the pustular stage of disease. In order to describe the kinetics of the host response, 23 subjects were infected at 2 sites with a standardized dose of H. ducreyi. Subjects were biopsied 1 or 4 days after inoculation or when they developed a painful pustular lesion (days 7-14). Papules and pustules contained a predominant T cell infiltrate that consisted of CD45RO and CD4 cells of the αβ lineage. Both papules and pustules contained mixed or T helper 1 type cytokine mRNA and interleukin-8 and tumor necrosis factor- α mRNA. Although the subjects had no history of chancroid, their immune responses resembled delayed-type hypersensitivity reactions that occurred within 24 h of inoculation and persisted throughout the course of experimental infection.

Original languageEnglish
Pages (from-to)1688-1697
Number of pages10
JournalJournal of Infectious Diseases
Volume178
Issue number6
DOIs
StatePublished - 1998

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Haemophilus ducreyi
Delayed Hypersensitivity
Chancroid
Messenger RNA
Cell Lineage
Infection
Interleukin-8
Tumor Necrosis Factor-alpha
Cytokines
T-Lymphocytes
Skin

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

The immune response to Haemophilus ducreyi resembles a delayed-type hypersensitivity reaction throughout experimental infection of human subjects. / Palmer, Katherine L.; Schnizlein-Bick, Carol T.; Orazi, Attilio; John, Karla; Chen, Cheng Yen; Hood, Antoinette F.; Spinola, Stanley.

In: Journal of Infectious Diseases, Vol. 178, No. 6, 1998, p. 1688-1697.

Research output: Contribution to journalArticle

Palmer, Katherine L. ; Schnizlein-Bick, Carol T. ; Orazi, Attilio ; John, Karla ; Chen, Cheng Yen ; Hood, Antoinette F. ; Spinola, Stanley. / The immune response to Haemophilus ducreyi resembles a delayed-type hypersensitivity reaction throughout experimental infection of human subjects. In: Journal of Infectious Diseases. 1998 ; Vol. 178, No. 6. pp. 1688-1697.
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