The immunoglobulin heavy-chain matrix-associating regions are bound by Bright: A B cell-specific trans-activator that describes a new DNA-binding protein family

Richard F. Herrscher, Mark Kaplan, David L. Lelsz, Chhaya Das, Richard Scheuermann, Philip W. Tucker

Research output: Contribution to journalArticle

214 Citations (Scopus)

Abstract

B lymphocyte-restricted transcription of immunoglobulin heavy-chain (IgH) genes is specified by elements within the variable region (V(H)) promoter and the intronic enhancer (Eμ). The gene encoding a protein that binds a V(H) promoter proximal site necessary for induced μ-heavy-chain transcription has been cloned. This B-cell specific protein, termed Bright (B cell regulator of IgH transcription), is found in both soluble and matrix insoluble nuclear fractions. Bright binds the minor groove of a restricted ATC sequence that is sufficient for nuclear matrix association. This sequence motif is present in previously described matrix-associating regions (MARs) proximal to the promoter and flanking Eμ. Bright can activate Eμ-driven transcription by binding these sites, but only when they occur in their natural context and in cell lines permissive for Eμ activity. To bind DNA, Bright requires a novel tetramerization domain and a previously undescribed domain that shares identity with several proteins, including SWI1, a component of the SWI/SNF complex.

Original languageEnglish (US)
Pages (from-to)3067-3082
Number of pages16
JournalGenes and Development
Volume9
Issue number24
StatePublished - Dec 15 1995
Externally publishedYes

Fingerprint

Immunoglobulin Heavy Chains
Trans-Activators
DNA-Binding Proteins
B-Lymphocytes
Nuclear Matrix
Immunoglobulin Heavy Chain Genes
Proteins
Binding Sites
Cell Line
DNA
Genes

Keywords

  • B lymphocyte
  • IgH enhancer
  • immunoglobulin transcription
  • MAR-binding protein
  • matrix-associating regions
  • Nuclear matrix

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

The immunoglobulin heavy-chain matrix-associating regions are bound by Bright : A B cell-specific trans-activator that describes a new DNA-binding protein family. / Herrscher, Richard F.; Kaplan, Mark; Lelsz, David L.; Das, Chhaya; Scheuermann, Richard; Tucker, Philip W.

In: Genes and Development, Vol. 9, No. 24, 15.12.1995, p. 3067-3082.

Research output: Contribution to journalArticle

Herrscher, Richard F. ; Kaplan, Mark ; Lelsz, David L. ; Das, Chhaya ; Scheuermann, Richard ; Tucker, Philip W. / The immunoglobulin heavy-chain matrix-associating regions are bound by Bright : A B cell-specific trans-activator that describes a new DNA-binding protein family. In: Genes and Development. 1995 ; Vol. 9, No. 24. pp. 3067-3082.
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