The impact of assay sensitivity in the assessment of diseases and disorders in children

Samar N. Rahhal, John S. Fuqua, Peter A. Lee

Research output: Contribution to journalArticle

16 Scopus citations


Accurate measurement of the low levels of testosterone (T) and estradiol (E2) present in normal children and in children with disorders of puberty and sexual development is critical both for appropriate diagnosis and treatment and for clinical research studies. However, measurement of these levels lacks needed precision because of inadequate sensitivity of most commercially available assays and poor accuracy at the low levels found in normal childhood and most disorders. While immunoassays presently do not appear to have the potential to provide more accurate measurements, isotope dilution-gas chromatography/mass spectrometry and liquid chromatography/tandem mass spectrometry techniques offer promise to meet this need to improve clinical care and research.

Original languageEnglish (US)
Pages (from-to)1322-1327
Number of pages6
Issue number13
StatePublished - Dec 12 2008


  • Enzyme-linked immunosorbent assays
  • Isotope dilution-gas chromatography/mass spectrometry
  • Liquid chromatography/tandem mass spectrometry
  • Radioimmunoassay
  • Steroid assays

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Molecular Biology
  • Organic Chemistry
  • Pharmacology

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