The impact of three strains of oral bacteria on the surface and mechanical properties of a dental resin material

Karen S. Gregson, Han Shih, Richard Gregory

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The purpose of this study was to determine if three strains of bacteria could impact the mechanical or surface properties of a dental resin material. Resin material specimens were incubated at 37°C in sterile saline, tryptic soy broth supplemented with sucrose (TSBS), or TSBS inoculated with Streptococcus mutans, Streptococcus gordonii, or Streptococcus sanguis. The specimens were subjected to Fourier transform infrared spectroscopy before and after incubation. The flexural strength test was performed once a week for 6 weeks. Microhardness and scanning electron microscopy (SEM) was performed on specimens at 1 and 6 weeks. Differences in the area under the carbonyl peak were statistically significant for the specimens incubated in the media inoculated with either S. mutans or S. gordonii. To determine why S. sanguis did not produce changes as the other bacteria did, triethylene glycol dimethacrylate, methacrylic acid, and triethylene glycol were added to bacterial cultures at increasing concentrations. Both methacrylic acid and triethylene glycol reduced the number of colony-forming units of S. sanguis. Specimens incubated in TSBS, saline or in culture with S. sanguis demonstrated a decrease in peak stress in week 1 of the flexure strength test. SEM demonstrated that surface topology changed for those specimens incubated in culture with S. mutans or S. gordonii. The changes in surface topology demonstrated here could contribute to the secondary caries and changes in esthetic properties seen clinically with the use of resin materials in dental restorations.

Original languageEnglish
Pages (from-to)1095-1103
Number of pages9
JournalClinical Oral Investigations
Volume16
Issue number4
DOIs
StatePublished - Aug 2012

Fingerprint

Synthetic Resins
Streptococcus sanguis
Dental Materials
Surface Properties
Streptococcus gordonii
Streptococcus mutans
Bacteria
Sucrose
Electron Scanning Microscopy
Fourier Transform Infrared Spectroscopy
Esthetics
Stem Cells
trypticase-soy broth
methacrylic acid
triethylene glycol

Keywords

  • Biodegradation
  • Dental materials
  • Oral bacteria

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

The impact of three strains of oral bacteria on the surface and mechanical properties of a dental resin material. / Gregson, Karen S.; Shih, Han; Gregory, Richard.

In: Clinical Oral Investigations, Vol. 16, No. 4, 08.2012, p. 1095-1103.

Research output: Contribution to journalArticle

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