The in-vitro response of human peripheral blood neutrophils to fusobacterium nucleatum

Vivian Y. Wahaidi, George J. Eckert, Richard Gregory, Michael Kowolik, Dominique M. Galli

Research output: Contribution to journalArticle

Abstract

Fusobacterium nucleatum is associated with periodontal disease and bacteremias of oral origin. The interaction between oral bacteria and host defense is central in linking oral and systemic diseases. Purpose: To evaluate the oxidative response of human peripheral blood neutrophils, in terms of priming and activation, to F. nucleatum. Methods: The oxidative activity of peripheral blood neutrophils was evaluated by luminol-enhanced chemiluminescence. Assays were conducted using F. nucleatum ATCC 10953. In addition, Staphylococcus aureus ATCC 9144 and Aggregatibacter actinomycetemcomitans ATCC 33384 were used as bacterial controls. All bacteria were killed by exposure to UV light prior to use in the assays. Comparisons among the groups were performed using ANOVA. Results: Activation of neutrophils with F. nucleatum resulted in significantly higher total chemiluminescence (p<0.05) when compared to A. actinomycetemcomitans and S. aureus. Priming of neutrophils with N-formyl-L-methionyl-L-leucyl-L-phenylalanine or F. nucleatum did not increase the chemiluminescence response. Clinical Significance: Despite the absence of a priming effect, the current study confirmed that F. nucleatum is a potent activator of peripheral blood neutrophils, thus increasing systemic levels of oxidative stress and risk for systemic disease.

Original languageEnglish
Pages (from-to)59-66
Number of pages8
JournalInternational Journal of Clinical Dentistry
Volume6
Issue number1
StatePublished - 2013

Fingerprint

Fusobacterium nucleatum
Neutrophils
Luminescence
Aggregatibacter actinomycetemcomitans
Staphylococcus aureus
leucyl-phenylalanine
Mouth Diseases
Bacteria
Luminol
Neutrophil Activation
Periodontal Diseases
Ultraviolet Rays
Bacteremia
Phenylalanine
In Vitro Techniques
Analysis of Variance
Oxidative Stress

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

The in-vitro response of human peripheral blood neutrophils to fusobacterium nucleatum. / Wahaidi, Vivian Y.; Eckert, George J.; Gregory, Richard; Kowolik, Michael; Galli, Dominique M.

In: International Journal of Clinical Dentistry, Vol. 6, No. 1, 2013, p. 59-66.

Research output: Contribution to journalArticle

@article{40f22cdce3d448dea51c4d7b310e5e1d,
title = "The in-vitro response of human peripheral blood neutrophils to fusobacterium nucleatum",
abstract = "Fusobacterium nucleatum is associated with periodontal disease and bacteremias of oral origin. The interaction between oral bacteria and host defense is central in linking oral and systemic diseases. Purpose: To evaluate the oxidative response of human peripheral blood neutrophils, in terms of priming and activation, to F. nucleatum. Methods: The oxidative activity of peripheral blood neutrophils was evaluated by luminol-enhanced chemiluminescence. Assays were conducted using F. nucleatum ATCC 10953. In addition, Staphylococcus aureus ATCC 9144 and Aggregatibacter actinomycetemcomitans ATCC 33384 were used as bacterial controls. All bacteria were killed by exposure to UV light prior to use in the assays. Comparisons among the groups were performed using ANOVA. Results: Activation of neutrophils with F. nucleatum resulted in significantly higher total chemiluminescence (p<0.05) when compared to A. actinomycetemcomitans and S. aureus. Priming of neutrophils with N-formyl-L-methionyl-L-leucyl-L-phenylalanine or F. nucleatum did not increase the chemiluminescence response. Clinical Significance: Despite the absence of a priming effect, the current study confirmed that F. nucleatum is a potent activator of peripheral blood neutrophils, thus increasing systemic levels of oxidative stress and risk for systemic disease.",
author = "Wahaidi, {Vivian Y.} and Eckert, {George J.} and Richard Gregory and Michael Kowolik and Galli, {Dominique M.}",
year = "2013",
language = "English",
volume = "6",
pages = "59--66",
journal = "International Journal of Clinical Dentistry",
issn = "1939-5833",
publisher = "Nova Science Publishers Inc",
number = "1",

}

TY - JOUR

T1 - The in-vitro response of human peripheral blood neutrophils to fusobacterium nucleatum

AU - Wahaidi, Vivian Y.

AU - Eckert, George J.

AU - Gregory, Richard

AU - Kowolik, Michael

AU - Galli, Dominique M.

PY - 2013

Y1 - 2013

N2 - Fusobacterium nucleatum is associated with periodontal disease and bacteremias of oral origin. The interaction between oral bacteria and host defense is central in linking oral and systemic diseases. Purpose: To evaluate the oxidative response of human peripheral blood neutrophils, in terms of priming and activation, to F. nucleatum. Methods: The oxidative activity of peripheral blood neutrophils was evaluated by luminol-enhanced chemiluminescence. Assays were conducted using F. nucleatum ATCC 10953. In addition, Staphylococcus aureus ATCC 9144 and Aggregatibacter actinomycetemcomitans ATCC 33384 were used as bacterial controls. All bacteria were killed by exposure to UV light prior to use in the assays. Comparisons among the groups were performed using ANOVA. Results: Activation of neutrophils with F. nucleatum resulted in significantly higher total chemiluminescence (p<0.05) when compared to A. actinomycetemcomitans and S. aureus. Priming of neutrophils with N-formyl-L-methionyl-L-leucyl-L-phenylalanine or F. nucleatum did not increase the chemiluminescence response. Clinical Significance: Despite the absence of a priming effect, the current study confirmed that F. nucleatum is a potent activator of peripheral blood neutrophils, thus increasing systemic levels of oxidative stress and risk for systemic disease.

AB - Fusobacterium nucleatum is associated with periodontal disease and bacteremias of oral origin. The interaction between oral bacteria and host defense is central in linking oral and systemic diseases. Purpose: To evaluate the oxidative response of human peripheral blood neutrophils, in terms of priming and activation, to F. nucleatum. Methods: The oxidative activity of peripheral blood neutrophils was evaluated by luminol-enhanced chemiluminescence. Assays were conducted using F. nucleatum ATCC 10953. In addition, Staphylococcus aureus ATCC 9144 and Aggregatibacter actinomycetemcomitans ATCC 33384 were used as bacterial controls. All bacteria were killed by exposure to UV light prior to use in the assays. Comparisons among the groups were performed using ANOVA. Results: Activation of neutrophils with F. nucleatum resulted in significantly higher total chemiluminescence (p<0.05) when compared to A. actinomycetemcomitans and S. aureus. Priming of neutrophils with N-formyl-L-methionyl-L-leucyl-L-phenylalanine or F. nucleatum did not increase the chemiluminescence response. Clinical Significance: Despite the absence of a priming effect, the current study confirmed that F. nucleatum is a potent activator of peripheral blood neutrophils, thus increasing systemic levels of oxidative stress and risk for systemic disease.

UR - http://www.scopus.com/inward/record.url?scp=84878046527&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878046527&partnerID=8YFLogxK

M3 - Article

VL - 6

SP - 59

EP - 66

JO - International Journal of Clinical Dentistry

JF - International Journal of Clinical Dentistry

SN - 1939-5833

IS - 1

ER -