Fusobacterium nucleatum is associated with periodontal disease and bacteremias of oral origin. The interaction between oral bacteria and host defense is central in linking oral and systemic diseases. Purpose: To evaluate the oxidative response of human peripheral blood neutrophils, in terms of priming and activation, to F. nucleatum. Methods: The oxidative activity of peripheral blood neutrophils was evaluated by luminol-enhanced chemiluminescence. Assays were conducted using F. nucleatum ATCC 10953. In addition, Staphylococcus aureus ATCC 9144 and Aggregatibacter actinomycetemcomitans ATCC 33384 were used as bacterial controls. All bacteria were killed by exposure to UV light prior to use in the assays. Comparisons among the groups were performed using ANOVA. Results: Activation of neutrophils with F. nucleatum resulted in significantly higher total chemiluminescence (p<0.05) when compared to A. actinomycetemcomitans and S. aureus. Priming of neutrophils with N-formyl-L-methionyl-L-leucyl-L-phenylalanine or F. nucleatum did not increase the chemiluminescence response. Clinical Significance: Despite the absence of a priming effect, the current study confirmed that F. nucleatum is a potent activator of peripheral blood neutrophils, thus increasing systemic levels of oxidative stress and risk for systemic disease.
|Original language||English (US)|
|Number of pages||8|
|Journal||International Journal of Clinical Dentistry|
|State||Published - May 28 2013|
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