The in-vitro response of human peripheral blood neutrophils to fusobacterium nucleatum

Vivian Y. Wahaidi, George J. Eckert, Richard Gregory, Michael Kowolik, Dominique M. Galli

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Fusobacterium nucleatum is associated with periodontal disease and bacteremias of oral origin. The interaction between oral bacteria and host defense is central in linking oral and systemic diseases. Purpose: To evaluate the oxidative response of human peripheral blood neutrophils, in terms of priming and activation, to F. nucleatum. Methods: The oxidative activity of peripheral blood neutrophils was evaluated by luminol-enhanced chemiluminescence. Assays were conducted using F. nucleatum ATCC 10953. In addition, Staphylococcus aureus ATCC 9144 and Aggregatibacter actinomycetemcomitans ATCC 33384 were used as bacterial controls. All bacteria were killed by exposure to UV light prior to use in the assays. Comparisons among the groups were performed using ANOVA. Results: Activation of neutrophils with F. nucleatum resulted in significantly higher total chemiluminescence (p

Original languageEnglish (US)
Title of host publicationOral Pathology and Oral Medicine
PublisherNova Science Publishers, Inc.
Pages59-66
Number of pages8
ISBN (Print)9781634824491, 9781634824484
StatePublished - Apr 1 2015

ASJC Scopus subject areas

  • Dentistry(all)

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    Wahaidi, V. Y., Eckert, G. J., Gregory, R., Kowolik, M., & Galli, D. M. (2015). The in-vitro response of human peripheral blood neutrophils to fusobacterium nucleatum. In Oral Pathology and Oral Medicine (pp. 59-66). Nova Science Publishers, Inc..