The induction of congenital spinal deformities in mice by maternal carbon monoxide exposure

Randall T. Loder, Michael J. Hernandez, Amy L. Lerner, David J. Winebrener, Steven A. Goldstein, Robert N. Hensinger, Chang Ying Liu, M. Anthony Schork

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Carbon monoxide (CO) has been shown to be teratogenic in mice. High altitude hypoxia has also been shown to induce congenital vertebral anomalies in mice. The purpose of this study was to investigate the effect of maternal hypoxia owing to CO exposure and the production of congenital spinal deformities in the offspring. Sixty DBA-1J mice were bred using polygamous timed breeding methods. Pregnant females were exposed to 200, 400, or 600 ppm CO using a custom-designed gas blender system. Seven-hour exposures were performed on day 8.5, 9.5, or 10.5 of the 21-day gestation cycle. The neonates were euthanized at birth; the specimens were fixed, eviscerated, and radiographed. Congenital spinal deformities were observed (wedge, hemi, fused, and missing vertebrae; fused ribs) and were located in all regions of the spine. There was a statistically significant difference in the number of spinal deformities between all groups, with no defects in the controls and a 77% incidence at 600 ppm (p < 0.0001). There was no apparent correlation between the time of exposure and defect location. The most sensitive time of gestation was 9.5 days. We identified an animal model of congenital spinal deformities that compares favorably with the evidence of human congenital spinal deformities in cases of maternal exposure to CO and other gas and chemical fumes.

Original languageEnglish (US)
Pages (from-to)662-666
Number of pages5
JournalJournal of Pediatric Orthopaedics
Volume20
Issue number5
DOIs
StatePublished - Sep 23 2000

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Keywords

  • Carbon monoxide
  • Congenital spinal deformity
  • Hypoxia
  • Mice

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Orthopedics and Sports Medicine

Cite this

Loder, R. T., Hernandez, M. J., Lerner, A. L., Winebrener, D. J., Goldstein, S. A., Hensinger, R. N., Liu, C. Y., & Schork, M. A. (2000). The induction of congenital spinal deformities in mice by maternal carbon monoxide exposure. Journal of Pediatric Orthopaedics, 20(5), 662-666. https://doi.org/10.1097/00004694-200009000-00022