The inhibition of mitochondrial energized processes by fluorescein mercuric acetate

Martin J. Lee, Robert Harris, T. Wakabayashi, David E. Green

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Fluorescein mercuric acetate (FMA) has been shown to be a potent inhibitor of energized processes in both beef heart mitochondria and ETPH particles. FMA reacts preferentially with a small number of specific sulfur atoms and inhibits the phosphate-dependent configurational transition. FMA enhances the anaerobic to aerobic pH changes observed in intact mitochondria and submitochondrial particles, and also enhances nonenergized swelling in 0·15 M sodium or potassium chloride. The results are interpreted in terms of a model whereby FMA, in reacting with the mitochondrion, modifies its conformation. The resulting conformational changes which occur upon energization are therefore different from those conformational changes which would occur in the absence of FMA. The net result of this process is the inhibition of some processes (e.g., oxidative phosphorylation, ATP-32Pi exchange, etc.) and the enhancement of other processes (the proton shift and nonenergized swelling in chloride salts).

Original languageEnglish (US)
Pages (from-to)13-31
Number of pages19
JournalJournal of Bioenergetics
Volume2
Issue number1
DOIs
StatePublished - Feb 1971
Externally publishedYes

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Mitochondria
Submitochondrial Particles
Heart Mitochondria
Potassium Chloride
Oxidative Phosphorylation
Sulfur
Sodium Chloride
Protons
Chlorides
Salts
Adenosine Triphosphate
Phosphates
fluorescein mercuric acetate
Red Meat

ASJC Scopus subject areas

  • Physiology
  • Cell Biology
  • Medicine(all)

Cite this

The inhibition of mitochondrial energized processes by fluorescein mercuric acetate. / Lee, Martin J.; Harris, Robert; Wakabayashi, T.; Green, David E.

In: Journal of Bioenergetics, Vol. 2, No. 1, 02.1971, p. 13-31.

Research output: Contribution to journalArticle

Lee, Martin J. ; Harris, Robert ; Wakabayashi, T. ; Green, David E. / The inhibition of mitochondrial energized processes by fluorescein mercuric acetate. In: Journal of Bioenergetics. 1971 ; Vol. 2, No. 1. pp. 13-31.
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