The interaction of laser energy with ureter tissues in a long term investigation

U. Stratmann, K. Schaarschmidt, R. R. Lehmann, A. Heinze, G. H. Willital, E. Unsold, Andrew Evan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This study investigates tissue responses after laser irradiation of the rabbit ureter, which serves as an experimental model for rectourogenital fistulae of children. Twenty-five rabbit ureters were irradiated intraluminally by a Nd:YAG laser 1320 nm (2 Watt, 20 seconds and 3 Watt, 8 seconds) via an applicator with radialsymmetrical light distribution. Immediately, 2 weeks, 4 weeks, 8 weeks, and 16 weeks after irradiation, the ureters were X-rayed with contrast solution and prepared for light and transmission electron microscopy. For the parameters employed, no apparent morphological differences could be observed. Immediately, the central laser zone showed a transmural thermonecrosis prevailed by cellular destruction, condensed ground substance and occlusion of most vascular lumina. Peripheral laser zones displayed urothelial vacuolations. Between 2 and 16 weeks, urothelial regeneration and ingrowth of granulation tissue caused a luminal stenosis or occlusion followed by transformation into scar tissue. In some peripheral laser zones, a hydroureter with marked luminal dilatation developed. We conclude that the ureter is occluded if the expanding force of the growing scar tissue exceeds the hydrostatic pressure of the obstructed urine. A laser occlusion of rectourogenital fistulae will be easier to achieve since fistula occlusion does not entail an obstruction of the urine flow.

Original languageEnglish (US)
Pages (from-to)805-816
Number of pages12
JournalScanning Microscopy
Volume9
Issue number3
StatePublished - 1995
Externally publishedYes

Fingerprint

occlusion
Tissue
Lasers
scars
urine
lasers
rabbits
interactions
energy
Applicators
irradiation
lumens
Granulation
Hydrostatic pressure
Laser beam effects
regeneration
hydrostatic pressure
destruction
YAG lasers
Irradiation

Keywords

  • healing process
  • hydroureter
  • immediate tissue response
  • luminal occlusion
  • Nd:YAG laser
  • rabbit ureter
  • radiology
  • rectourogenital fistula
  • thermal necrosis
  • ultrastructure

ASJC Scopus subject areas

  • Instrumentation

Cite this

Stratmann, U., Schaarschmidt, K., Lehmann, R. R., Heinze, A., Willital, G. H., Unsold, E., & Evan, A. (1995). The interaction of laser energy with ureter tissues in a long term investigation. Scanning Microscopy, 9(3), 805-816.

The interaction of laser energy with ureter tissues in a long term investigation. / Stratmann, U.; Schaarschmidt, K.; Lehmann, R. R.; Heinze, A.; Willital, G. H.; Unsold, E.; Evan, Andrew.

In: Scanning Microscopy, Vol. 9, No. 3, 1995, p. 805-816.

Research output: Contribution to journalArticle

Stratmann, U, Schaarschmidt, K, Lehmann, RR, Heinze, A, Willital, GH, Unsold, E & Evan, A 1995, 'The interaction of laser energy with ureter tissues in a long term investigation', Scanning Microscopy, vol. 9, no. 3, pp. 805-816.
Stratmann U, Schaarschmidt K, Lehmann RR, Heinze A, Willital GH, Unsold E et al. The interaction of laser energy with ureter tissues in a long term investigation. Scanning Microscopy. 1995;9(3):805-816.
Stratmann, U. ; Schaarschmidt, K. ; Lehmann, R. R. ; Heinze, A. ; Willital, G. H. ; Unsold, E. ; Evan, Andrew. / The interaction of laser energy with ureter tissues in a long term investigation. In: Scanning Microscopy. 1995 ; Vol. 9, No. 3. pp. 805-816.
@article{e70272caf7d148f482c0bfdd2d0de7ed,
title = "The interaction of laser energy with ureter tissues in a long term investigation",
abstract = "This study investigates tissue responses after laser irradiation of the rabbit ureter, which serves as an experimental model for rectourogenital fistulae of children. Twenty-five rabbit ureters were irradiated intraluminally by a Nd:YAG laser 1320 nm (2 Watt, 20 seconds and 3 Watt, 8 seconds) via an applicator with radialsymmetrical light distribution. Immediately, 2 weeks, 4 weeks, 8 weeks, and 16 weeks after irradiation, the ureters were X-rayed with contrast solution and prepared for light and transmission electron microscopy. For the parameters employed, no apparent morphological differences could be observed. Immediately, the central laser zone showed a transmural thermonecrosis prevailed by cellular destruction, condensed ground substance and occlusion of most vascular lumina. Peripheral laser zones displayed urothelial vacuolations. Between 2 and 16 weeks, urothelial regeneration and ingrowth of granulation tissue caused a luminal stenosis or occlusion followed by transformation into scar tissue. In some peripheral laser zones, a hydroureter with marked luminal dilatation developed. We conclude that the ureter is occluded if the expanding force of the growing scar tissue exceeds the hydrostatic pressure of the obstructed urine. A laser occlusion of rectourogenital fistulae will be easier to achieve since fistula occlusion does not entail an obstruction of the urine flow.",
keywords = "healing process, hydroureter, immediate tissue response, luminal occlusion, Nd:YAG laser, rabbit ureter, radiology, rectourogenital fistula, thermal necrosis, ultrastructure",
author = "U. Stratmann and K. Schaarschmidt and Lehmann, {R. R.} and A. Heinze and Willital, {G. H.} and E. Unsold and Andrew Evan",
year = "1995",
language = "English (US)",
volume = "9",
pages = "805--816",
journal = "Scanning Microscopy",
issn = "0891-7035",
publisher = "Scanning Microscopy International",
number = "3",

}

TY - JOUR

T1 - The interaction of laser energy with ureter tissues in a long term investigation

AU - Stratmann, U.

AU - Schaarschmidt, K.

AU - Lehmann, R. R.

AU - Heinze, A.

AU - Willital, G. H.

AU - Unsold, E.

AU - Evan, Andrew

PY - 1995

Y1 - 1995

N2 - This study investigates tissue responses after laser irradiation of the rabbit ureter, which serves as an experimental model for rectourogenital fistulae of children. Twenty-five rabbit ureters were irradiated intraluminally by a Nd:YAG laser 1320 nm (2 Watt, 20 seconds and 3 Watt, 8 seconds) via an applicator with radialsymmetrical light distribution. Immediately, 2 weeks, 4 weeks, 8 weeks, and 16 weeks after irradiation, the ureters were X-rayed with contrast solution and prepared for light and transmission electron microscopy. For the parameters employed, no apparent morphological differences could be observed. Immediately, the central laser zone showed a transmural thermonecrosis prevailed by cellular destruction, condensed ground substance and occlusion of most vascular lumina. Peripheral laser zones displayed urothelial vacuolations. Between 2 and 16 weeks, urothelial regeneration and ingrowth of granulation tissue caused a luminal stenosis or occlusion followed by transformation into scar tissue. In some peripheral laser zones, a hydroureter with marked luminal dilatation developed. We conclude that the ureter is occluded if the expanding force of the growing scar tissue exceeds the hydrostatic pressure of the obstructed urine. A laser occlusion of rectourogenital fistulae will be easier to achieve since fistula occlusion does not entail an obstruction of the urine flow.

AB - This study investigates tissue responses after laser irradiation of the rabbit ureter, which serves as an experimental model for rectourogenital fistulae of children. Twenty-five rabbit ureters were irradiated intraluminally by a Nd:YAG laser 1320 nm (2 Watt, 20 seconds and 3 Watt, 8 seconds) via an applicator with radialsymmetrical light distribution. Immediately, 2 weeks, 4 weeks, 8 weeks, and 16 weeks after irradiation, the ureters were X-rayed with contrast solution and prepared for light and transmission electron microscopy. For the parameters employed, no apparent morphological differences could be observed. Immediately, the central laser zone showed a transmural thermonecrosis prevailed by cellular destruction, condensed ground substance and occlusion of most vascular lumina. Peripheral laser zones displayed urothelial vacuolations. Between 2 and 16 weeks, urothelial regeneration and ingrowth of granulation tissue caused a luminal stenosis or occlusion followed by transformation into scar tissue. In some peripheral laser zones, a hydroureter with marked luminal dilatation developed. We conclude that the ureter is occluded if the expanding force of the growing scar tissue exceeds the hydrostatic pressure of the obstructed urine. A laser occlusion of rectourogenital fistulae will be easier to achieve since fistula occlusion does not entail an obstruction of the urine flow.

KW - healing process

KW - hydroureter

KW - immediate tissue response

KW - luminal occlusion

KW - Nd:YAG laser

KW - rabbit ureter

KW - radiology

KW - rectourogenital fistula

KW - thermal necrosis

KW - ultrastructure

UR - http://www.scopus.com/inward/record.url?scp=0029585717&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029585717&partnerID=8YFLogxK

M3 - Article

VL - 9

SP - 805

EP - 816

JO - Scanning Microscopy

JF - Scanning Microscopy

SN - 0891-7035

IS - 3

ER -