The interactome of the atypical phosphatase Rtr1 in Saccharomyces cerevisiae

Whitney R. Smith-Kinnaman, Michael J. Berna, Gerald O. Hunter, Jason D. True, Peter Hsu, Gabriela I. Cabello, Melanie J. Fox, Gabriele Varani, Amber Mosley

Research output: Contribution to journalArticle

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Abstract

The phosphatase Rtr1 has been implicated in dephosphorylation of the RNA Polymerase II (RNAPII) C-terminal domain (CTD) during transcription elongation and in regulation of nuclear import of RNAPII. Although it has been shown that Rtr1 interacts with RNAPII in yeast and humans, the specific mechanisms that underlie Rtr1 recruitment to RNAPII have not been elucidated. To address this, we have performed an in-depth proteomic analysis of Rtr1 interacting proteins in yeast. Our studies revealed that hyperphosphorylated RNAPII is the primary interacting partner for Rtr1. To extend these findings, we performed quantitative proteomic analyses of Rtr1 interactions in yeast strains deleted for CTK1, the gene encoding the catalytic subunit of the CTD kinase I (CTDK-I) complex. Interestingly, we found that the interaction between Rtr1 and RNAPII is decreased in ctk1Δ strains. We hypothesize that serine-2 CTD phosphorylation is required for Rtr1 recruitment to RNAPII during transcription elongation. This journal is

Original languageEnglish
Pages (from-to)1730-1741
Number of pages12
JournalMolecular BioSystems
Volume10
Issue number7
DOIs
StatePublished - 2014

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RNA Polymerase II
Phosphoric Monoester Hydrolases
Saccharomyces cerevisiae
Proteomics
Yeasts
RNA Polymerase III
Nuclear RNA
Fungal Proteins
Cell Nucleus Active Transport
Serine
Catalytic Domain
Phosphotransferases
Phosphorylation
Genes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Medicine(all)

Cite this

Smith-Kinnaman, W. R., Berna, M. J., Hunter, G. O., True, J. D., Hsu, P., Cabello, G. I., ... Mosley, A. (2014). The interactome of the atypical phosphatase Rtr1 in Saccharomyces cerevisiae. Molecular BioSystems, 10(7), 1730-1741. https://doi.org/10.1039/c4mb00109e

The interactome of the atypical phosphatase Rtr1 in Saccharomyces cerevisiae. / Smith-Kinnaman, Whitney R.; Berna, Michael J.; Hunter, Gerald O.; True, Jason D.; Hsu, Peter; Cabello, Gabriela I.; Fox, Melanie J.; Varani, Gabriele; Mosley, Amber.

In: Molecular BioSystems, Vol. 10, No. 7, 2014, p. 1730-1741.

Research output: Contribution to journalArticle

Smith-Kinnaman, WR, Berna, MJ, Hunter, GO, True, JD, Hsu, P, Cabello, GI, Fox, MJ, Varani, G & Mosley, A 2014, 'The interactome of the atypical phosphatase Rtr1 in Saccharomyces cerevisiae', Molecular BioSystems, vol. 10, no. 7, pp. 1730-1741. https://doi.org/10.1039/c4mb00109e
Smith-Kinnaman WR, Berna MJ, Hunter GO, True JD, Hsu P, Cabello GI et al. The interactome of the atypical phosphatase Rtr1 in Saccharomyces cerevisiae. Molecular BioSystems. 2014;10(7):1730-1741. https://doi.org/10.1039/c4mb00109e
Smith-Kinnaman, Whitney R. ; Berna, Michael J. ; Hunter, Gerald O. ; True, Jason D. ; Hsu, Peter ; Cabello, Gabriela I. ; Fox, Melanie J. ; Varani, Gabriele ; Mosley, Amber. / The interactome of the atypical phosphatase Rtr1 in Saccharomyces cerevisiae. In: Molecular BioSystems. 2014 ; Vol. 10, No. 7. pp. 1730-1741.
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