The interactome of the atypical phosphatase Rtr1 in Saccharomyces cerevisiae

Whitney R. Smith-Kinnaman, Michael J. Berna, Gerald O. Hunter, Jason D. True, Peter Hsu, Gabriela I. Cabello, Melanie J. Fox, Gabriele Varani, Amber L. Mosley

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

The phosphatase Rtr1 has been implicated in dephosphorylation of the RNA Polymerase II (RNAPII) C-terminal domain (CTD) during transcription elongation and in regulation of nuclear import of RNAPII. Although it has been shown that Rtr1 interacts with RNAPII in yeast and humans, the specific mechanisms that underlie Rtr1 recruitment to RNAPII have not been elucidated. To address this, we have performed an in-depth proteomic analysis of Rtr1 interacting proteins in yeast. Our studies revealed that hyperphosphorylated RNAPII is the primary interacting partner for Rtr1. To extend these findings, we performed quantitative proteomic analyses of Rtr1 interactions in yeast strains deleted for CTK1, the gene encoding the catalytic subunit of the CTD kinase I (CTDK-I) complex. Interestingly, we found that the interaction between Rtr1 and RNAPII is decreased in ctk1Δ strains. We hypothesize that serine-2 CTD phosphorylation is required for Rtr1 recruitment to RNAPII during transcription elongation. This journal is

Original languageEnglish (US)
Pages (from-to)1730-1741
Number of pages12
JournalMolecular BioSystems
Volume10
Issue number7
DOIs
StatePublished - Jul 2014

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

Fingerprint Dive into the research topics of 'The interactome of the atypical phosphatase Rtr1 in Saccharomyces cerevisiae'. Together they form a unique fingerprint.

  • Cite this

    Smith-Kinnaman, W. R., Berna, M. J., Hunter, G. O., True, J. D., Hsu, P., Cabello, G. I., Fox, M. J., Varani, G., & Mosley, A. L. (2014). The interactome of the atypical phosphatase Rtr1 in Saccharomyces cerevisiae. Molecular BioSystems, 10(7), 1730-1741. https://doi.org/10.1039/c4mb00109e