The interphase microtubule damage checkpoint defines an S-phase commitment point and does not require p21waf-1

Charlie R. Mantel, Stephen E. Braun, Younghee Lee, Young June Kim, Hal Broxmeyer

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Cell cycle checkpoints ensure orderly progression of events during cell division. A microtubule damage (MTD)-induced checkpoint has been described in G1 phase of the cell cycle (G1MTC) for which little is known. The present study shows that the G1MTC is intact in activated T lymphocytes from mice with the p21waf-1 gene deleted. However, p21waf-1 gene deletion does affect the ratio of cells that arrest at the G1MTC and the spindle checkpoint after MTD. The G1MTC arrests T lymphocytes in G1 prior to cdc2 up-regulation and prior to G1 arrest by p21waf-1. Once cells have progressed past the G1MTC, they are committed to chromosome replication and metaphase progression, even with extreme MTD. The G1MTC is also present in a human myeloid cell line deficient in p21waf-1 gene expression. The p21-independent G1MTC may be important in cellular responses to MTD such as those induced by drugs used to treat cancer.

Original languageEnglish
Pages (from-to)1505-1507
Number of pages3
JournalBlood
Volume97
Issue number5
DOIs
StatePublished - Mar 1 2001

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S Phase Cell Cycle Checkpoints
Interphase
Microtubules
Cells
T-cells
Genes
T-Lymphocytes
Gene Deletion
G1 Phase
Myeloid Cells
Metaphase
Chromosomes
Cell Cycle Checkpoints
Gene expression
Cell Division
Cell Cycle
Up-Regulation
Gene Expression
Cell Line
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Hematology

Cite this

The interphase microtubule damage checkpoint defines an S-phase commitment point and does not require p21waf-1. / Mantel, Charlie R.; Braun, Stephen E.; Lee, Younghee; Kim, Young June; Broxmeyer, Hal.

In: Blood, Vol. 97, No. 5, 01.03.2001, p. 1505-1507.

Research output: Contribution to journalArticle

Mantel, Charlie R. ; Braun, Stephen E. ; Lee, Younghee ; Kim, Young June ; Broxmeyer, Hal. / The interphase microtubule damage checkpoint defines an S-phase commitment point and does not require p21waf-1. In: Blood. 2001 ; Vol. 97, No. 5. pp. 1505-1507.
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