The intra-oral effect on enamel demineralization of extracellular matrix material synthesized from sucrose by Streptococcus mutans.

Domenick Zero, J. van Houte, J. Russo

Research output: Contribution to journalArticle

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Abstract

The role of extracellular matrix material (EMM) synthesized from sucrose (S) by Streptococcus mutans IB-1600 in altering the demineralizing potential of artificial plaque was evaluated with an intraoral enamel demineralization test (IEDT). The artificial plaque samples were prepared from cells cultivated in Todd-Hewitt broth (THB) supplemented with various S concentrations and by mixing THB-grown cells with increasing proportions of EMM (heat-killed THB + 2% S-cultivated cells). The samples were also evaluated for cell density (DNA content) and acidogenicity in vitro (pH-stat), as well as for in situ pH changes during a 45-minute intra-oral test following a 10% glucose rinse. An increase in the proportion of EMM relative to cell density was associated with an increase in enamel demineralization. This trend reversed when the ratio of cells to EMM was less than 1:19. Experiments involving strains of S. mitis, S. sanguis, and S. salivarius suggested a similar effect of EMM. The intra-oral pH data suggest that the presence of EMM may enhance demineralization by altering diffusion properties of plaque.

Original languageEnglish
Pages (from-to)918-923
Number of pages6
JournalJournal of Dental Research
Volume65
Issue number6
StatePublished - Jun 1986
Externally publishedYes

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Streptococcus mutans
Dental Enamel
Extracellular Matrix
Sucrose
Cell Count
Hot Temperature
Glucose
DNA

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

The intra-oral effect on enamel demineralization of extracellular matrix material synthesized from sucrose by Streptococcus mutans. / Zero, Domenick; van Houte, J.; Russo, J.

In: Journal of Dental Research, Vol. 65, No. 6, 06.1986, p. 918-923.

Research output: Contribution to journalArticle

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