The lipidomic analyses in low and highly aggressive ovarian cancer cell lines

Zhenwen Zhao, Qingchun Cai, Yan Xu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Despite huge advances in the research of epithelial ovarian cancer (EOC), it remains the most lethal gynecological malignancy. Peritoneal tumor cell dissemination with cell survival and drug-resistance to taxane and platinum-based chemotherapy are two of the major challenges of EOC treatment. We have generated highly aggressive EOC cell lines (ID8-P1 lines or P1) from ID8-P0 (without in vivo passage, or P0) through in vivo passage in mice. We conducted lipidomic analyses in cells from ID8-P0 versus three ID8-P1 cell lines using ultra-high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry. A total of 16 classes of lipids (149 individual lipids) were analyzed and compared between P0 and P1 cells. In addition to overall lipid profiles in EOC cells, we had several novel observations. Several classes and species of lipids have been identified to be differentially present in P0 versus P1 cells, which are potentially involved in the acquired aggressiveness of P1 cells. Triacylglycerols (TAG) were dramatically increased under detachment stress in EOC cells. Since survival of EOC cells under detachment is one of the major obstacles for EOC treatment, further studies identifying the molecular mechanisms controlling TAG increase may lead to new treatment modalities for EOC.

Original languageEnglish (US)
Pages (from-to)179-187
Number of pages9
JournalLipids
Volume51
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

Ovarian Neoplasms
Cells
Lipids
Cell Line
Oncology
Triglycerides
Electrospray ionization
Chemotherapy
High performance liquid chromatography
Platinum
Mass spectrometry
Tumors
Electrospray Ionization Mass Spectrometry
Ovarian epithelial cancer
Tandem Mass Spectrometry
Drug Resistance
Pharmaceutical Preparations
Neoplasms
Cell Survival
Therapeutics

Keywords

  • Electrospray ionization-mass spectrometry (ESI-MS)
  • Epithelial ovarian cancer (EOC)
  • ID8
  • Lipidomic analyses
  • Triacylglycerol (TAG)

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Organic Chemistry

Cite this

The lipidomic analyses in low and highly aggressive ovarian cancer cell lines. / Zhao, Zhenwen; Cai, Qingchun; Xu, Yan.

In: Lipids, Vol. 51, No. 2, 01.02.2016, p. 179-187.

Research output: Contribution to journalArticle

Zhao, Zhenwen ; Cai, Qingchun ; Xu, Yan. / The lipidomic analyses in low and highly aggressive ovarian cancer cell lines. In: Lipids. 2016 ; Vol. 51, No. 2. pp. 179-187.
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