The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene

Michael J. Klemsz, Scott R. McKercher, Antonio Celada, Charles Van Beveren, Richard A. Maki

Research output: Contribution to journalArticle

719 Scopus citations

Abstract

We have isolated a cDNA clone, PU.1, that codes for a new tissue-specific DNA binding protein. Analysis of the binding site by methylation interference and DNA-ase I protection revealed that the PU.1 protein recognized a purine-rich sequence, 5′-GAGGAA-3′ (PU box). The PU.1 protein was shown to be a transcriptional activator that is expressed in macrophages and B cells. cDNA constructions used to generate proteins lacking portions of either the amino- or carboxy-terminal ends of the PU.1 protein placed the DNA binding domain in the highly basic carboxy-terminal domain of the protein. The amino acid sequence in the binding domain of PU.1 has considerable identity with proteins belonging to the ets oncogene family.

Original languageEnglish (US)
Pages (from-to)113-124
Number of pages12
JournalCell
Volume61
Issue number1
DOIs
StatePublished - Apr 6 1990
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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