The macrophage mediates the renoprotective effects of endotoxin preconditioning

Takashi Hato, Seth Winfree, Rabih Kalakeche, Shataakshi Dube, Rakesh Kumar, Momoko Yoshimoto, Zoya Plotkin, Pierre Dagher

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Preconditioning is a preventative approach, whereby minimized insults generate protection against subsequent larger exposures to the same or even different insults. In immune cells, endotoxin preconditioning downregulates the inflammatory response and yet, preserves the ability to contain infections. However, the protective mechanisms of preconditioning at the tissue level in organs such as the kidney remain poorly understood. Here, we show that endotoxin preconditioning confers renal epithelial protection in various models of sepsis in vivo. We also tested the hypothesis that this protection results from direct interactions between the preconditioning dose of endotoxin and the renal tubules. This hypothesis is on the basis of our previous findings that endotoxin toxicity to nonpreconditioned renal tubules was direct and independent of immune cells. Notably, we found that tubular protection after preconditioning has an absolute requirement for CD14-expressing myeloid cells and particularly, macrophages. Additionally, an intact macrophage CD14-TRIF signaling pathway was essential for tubular protection. The preconditioned state was characterized by increased macrophage number and trafficking within the kidney as well as clustering of macrophages around S1 proximal tubules. These macrophages exhibited increased M2 polarization and upregulation of redox and iron-handling molecules. In renal tubules, preconditioning prevented peroxisomal damage and abolished oxidative stress and injury to S2 and S3 tubules. In summary, these data suggest that macrophages are essential mediators of endotoxin preconditioning and required for renal tissue protection. Preconditioning is, therefore, an attractive model to investigate novel protective pathways for the prevention and treatment of sepsis.

Original languageEnglish
Pages (from-to)1347-1362
Number of pages16
JournalJournal of the American Society of Nephrology
Volume26
Issue number6
DOIs
StatePublished - Jun 1 2015

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Endotoxins
Macrophages
Kidney
Sepsis
Myeloid Cells
Oxidation-Reduction
Cluster Analysis
Oxidative Stress
Up-Regulation
Down-Regulation
Iron
Wounds and Injuries
Infection

ASJC Scopus subject areas

  • Nephrology

Cite this

The macrophage mediates the renoprotective effects of endotoxin preconditioning. / Hato, Takashi; Winfree, Seth; Kalakeche, Rabih; Dube, Shataakshi; Kumar, Rakesh; Yoshimoto, Momoko; Plotkin, Zoya; Dagher, Pierre.

In: Journal of the American Society of Nephrology, Vol. 26, No. 6, 01.06.2015, p. 1347-1362.

Research output: Contribution to journalArticle

Hato, T, Winfree, S, Kalakeche, R, Dube, S, Kumar, R, Yoshimoto, M, Plotkin, Z & Dagher, P 2015, 'The macrophage mediates the renoprotective effects of endotoxin preconditioning', Journal of the American Society of Nephrology, vol. 26, no. 6, pp. 1347-1362. https://doi.org/10.1681/ASN.2014060561
Hato, Takashi ; Winfree, Seth ; Kalakeche, Rabih ; Dube, Shataakshi ; Kumar, Rakesh ; Yoshimoto, Momoko ; Plotkin, Zoya ; Dagher, Pierre. / The macrophage mediates the renoprotective effects of endotoxin preconditioning. In: Journal of the American Society of Nephrology. 2015 ; Vol. 26, No. 6. pp. 1347-1362.
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