The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein

Ping Lin, Helen Le-Niculescu, Robert Hofmeister, J. Michael McCaffery, Mingjie Jin, Hanjo Hennemann, Tammie McQuistan, Luc De Vries, Marilyn Gist Farquhar

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

We have identified CALNUC, an EF-hand, Ca2+-binding protein, as a Golgi resident protein. CALNUC corresponds to a previously identified EF- hand/calcium-binding protein known as nucleobindin. CALNUC interacts with Gα(i3) subunits in the yeast two-hybrid system and in GST-CALNUC pull-down assays. Analysis of deletion mutants demonstrated that the EF-hand and intervening acidic regions are the site of CALNUC's interaction with Gα(i3). CALNUC is found in both cytosolic and membrane fractions. The membrane pool is tightly associated with the luminal surface of Golgi membranes. CALNUC is widely expressed, as it is detected by immunofluorescence in the Golgi region of all tissues and cell lines examined. By immunoelectron microscopy, CALNUC is localized to cis-Golgi cisternae and the cis-Golgi network (CGN). CALNUC is the major Ca2+-binding protein detected by 45Ca2+-binding assay on Golgi fractions. The properties of CALNUC and its high homology to calreticulin suggest that it may play a key role in calcium homeostasis in the CGN and cis-Golgi cisternae.

Original languageEnglish (US)
Pages (from-to)1515-1527
Number of pages13
JournalJournal of Cell Biology
Volume141
Issue number7
DOIs
StatePublished - Jun 29 1998
Externally publishedYes

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Calcium-Binding Proteins
Proteins
EF Hand Motifs
Membranes
Carrier Proteins
nucleobindin
Calreticulin
Two-Hybrid System Techniques
Immunoelectron Microscopy
Fluorescent Antibody Technique
Homeostasis
Calcium

ASJC Scopus subject areas

  • Cell Biology

Cite this

Lin, P., Le-Niculescu, H., Hofmeister, R., McCaffery, J. M., Jin, M., Hennemann, H., ... Farquhar, M. G. (1998). The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein. Journal of Cell Biology, 141(7), 1515-1527. https://doi.org/10.1083/jcb.141.7.1515

The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein. / Lin, Ping; Le-Niculescu, Helen; Hofmeister, Robert; McCaffery, J. Michael; Jin, Mingjie; Hennemann, Hanjo; McQuistan, Tammie; De Vries, Luc; Farquhar, Marilyn Gist.

In: Journal of Cell Biology, Vol. 141, No. 7, 29.06.1998, p. 1515-1527.

Research output: Contribution to journalArticle

Lin, P, Le-Niculescu, H, Hofmeister, R, McCaffery, JM, Jin, M, Hennemann, H, McQuistan, T, De Vries, L & Farquhar, MG 1998, 'The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein', Journal of Cell Biology, vol. 141, no. 7, pp. 1515-1527. https://doi.org/10.1083/jcb.141.7.1515
Lin, Ping ; Le-Niculescu, Helen ; Hofmeister, Robert ; McCaffery, J. Michael ; Jin, Mingjie ; Hennemann, Hanjo ; McQuistan, Tammie ; De Vries, Luc ; Farquhar, Marilyn Gist. / The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein. In: Journal of Cell Biology. 1998 ; Vol. 141, No. 7. pp. 1515-1527.
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