The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein

Ping Lin, Helen Le-Niculescu, Robert Hofmeister, J. Michael McCaffery, Mingjie Jin, Hanjo Hennemann, Tammie McQuistan, Luc De Vries, Marilyn Gist Farquhar

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129 Scopus citations


We have identified CALNUC, an EF-hand, Ca2+-binding protein, as a Golgi resident protein. CALNUC corresponds to a previously identified EF- hand/calcium-binding protein known as nucleobindin. CALNUC interacts with Gα(i3) subunits in the yeast two-hybrid system and in GST-CALNUC pull-down assays. Analysis of deletion mutants demonstrated that the EF-hand and intervening acidic regions are the site of CALNUC's interaction with Gα(i3). CALNUC is found in both cytosolic and membrane fractions. The membrane pool is tightly associated with the luminal surface of Golgi membranes. CALNUC is widely expressed, as it is detected by immunofluorescence in the Golgi region of all tissues and cell lines examined. By immunoelectron microscopy, CALNUC is localized to cis-Golgi cisternae and the cis-Golgi network (CGN). CALNUC is the major Ca2+-binding protein detected by 45Ca2+-binding assay on Golgi fractions. The properties of CALNUC and its high homology to calreticulin suggest that it may play a key role in calcium homeostasis in the CGN and cis-Golgi cisternae.

Original languageEnglish (US)
Pages (from-to)1515-1527
Number of pages13
JournalJournal of Cell Biology
Issue number7
StatePublished - Jun 29 1998
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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    Lin, P., Le-Niculescu, H., Hofmeister, R., McCaffery, J. M., Jin, M., Hennemann, H., McQuistan, T., De Vries, L., & Farquhar, M. G. (1998). The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein. Journal of Cell Biology, 141(7), 1515-1527.