The matricellular protein periostin is required for sost inhibition and the anabolic response to mechanical loading and physical activity

Nicolas Bonnet, Kara N. Standley, Estelle N. Bianchi, Vincent Stadelmann, Michelangelo Foti, Simon Conway, Serge L. Ferrari

Research output: Contribution to journalArticle

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Abstract

Periostin (gene Postn) is a secreted extracellular matrix protein involved in cell recruitment and adhesion and plays an important role in odontogenesis. In bone, periostin is preferentially expressed in the periosteum, but its functional significance remains unclear. We investigated Postn-/- mice and their wild type littermates to elucidate the role of periostin in the skeletal response to moderate physical activity and direct axial compression of the tibia. Furthermore, we administered a sclerostinblocking antibody to these mice in order to demonstrate the influence of sustained Sost expression in their altered bone phenotypes. Cancellous and cortical bone microarchitecture as well as bending strength were altered in Postn-/ - compared with Postnb/b mice. Exercise and axial compression both significantly increased bone mineral density and trabecular and cortical microarchitecture as well as biomechanical properties of the long bones in Postn+/+ mice by increasing the bone formation activity, particularly at the periosteum. These changes correlated with an increase of periostin expression and a consecutive decrease of Sost in the stimulated bones. In contrast, mechanical stimuli had no effect on the skeletal properties of Postn-/- mice, where base-line expression of Sost levels were higher than Postn+/+ and remained unchanged following axial compression. In turn, the concomitant injection of sclerostin-blocking antibody rescued the bone biomechanical response in Postn-/- mice. Taken together, these results indicate that the matricellular periostin protein is required for Sost inhibition and thereby plays an important role in the determination of bone mass and microstructural in response to loading.

Original languageEnglish
Pages (from-to)35939-35950
Number of pages12
JournalJournal of Biological Chemistry
Volume284
Issue number51
DOIs
StatePublished - Dec 18 2009

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Bone
Bone and Bones
Periosteum
Proteins
Axial compression
Odontogenesis
Blocking Antibodies
Extracellular Matrix Proteins
Tibia
Osteogenesis
Cell Adhesion
Bone Density
Phenotype
Bending strength
Injections
Minerals
Antibodies
Adhesion
Genes

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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The matricellular protein periostin is required for sost inhibition and the anabolic response to mechanical loading and physical activity. / Bonnet, Nicolas; Standley, Kara N.; Bianchi, Estelle N.; Stadelmann, Vincent; Foti, Michelangelo; Conway, Simon; Ferrari, Serge L.

In: Journal of Biological Chemistry, Vol. 284, No. 51, 18.12.2009, p. 35939-35950.

Research output: Contribution to journalArticle

Bonnet, Nicolas ; Standley, Kara N. ; Bianchi, Estelle N. ; Stadelmann, Vincent ; Foti, Michelangelo ; Conway, Simon ; Ferrari, Serge L. / The matricellular protein periostin is required for sost inhibition and the anabolic response to mechanical loading and physical activity. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 51. pp. 35939-35950.
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