The Matrix Attachment Region-binding Protein SATB1 Interacts with Multiple Elements within the gp91phox Promoter and Is Down-regulated during Myeloid Differentiation

Shannon Hawkins, Terumi Kohwi-Shigematsu, David G. Skalnik

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The gp91phox gene encodes a component of the respiratory burst NADPH oxidase complex and is highly expressed in mature myeloid cells. The transcriptional repressor CCAAT displacement protein binds to at least five sites within the proximal gp91phox promoter and represses expression prior to terminal phagocyte differentiation. The DNA binding activity of CCAAT displacement protein decreases during terminal phagocyte differentiation, thus permitting the binding of transcriptional activators and induction of gp91 phox expression. We report here that the matrix attachment region-binding protein SATB1 interacts with at least seven sites within the -1542 to +12-base pair gp91phox promoter. Four additional binding sites for CCAAT displacement protein were also identified. Furthermore, the most proximal SATB1-binding site within the gp91phox promoter binds specifically to the nuclear matrix fraction in vitro. SATB1 expression is down-regulated during terminal myeloid cell differentiation, coincident with induction of gp91phox expression. Transient transfection assays demonstrate that a SATB1-binding site derived from the gp91phox promoter represses promoter activity in cells expressing SATB1. These findings underscore the importance of transcriptional repression in the regulation of gp91phox expression and reveal a candidate myeloid cell target gene for SATB1, a factor previously found to be essential for T cell development.

Original languageEnglish (US)
Pages (from-to)44472-44480
Number of pages9
JournalJournal of Biological Chemistry
Volume276
Issue number48
DOIs
StatePublished - Nov 30 2001

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Matrix Attachment Region Binding Proteins
Myeloid Cells
Binding Sites
Phagocytes
Genes
Nuclear Matrix
Proteins
T-cells
NADPH Oxidase
Base Pairing
Transfection
Cell Differentiation
Assays
Cells
T-Lymphocytes
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

The Matrix Attachment Region-binding Protein SATB1 Interacts with Multiple Elements within the gp91phox Promoter and Is Down-regulated during Myeloid Differentiation. / Hawkins, Shannon; Kohwi-Shigematsu, Terumi; Skalnik, David G.

In: Journal of Biological Chemistry, Vol. 276, No. 48, 30.11.2001, p. 44472-44480.

Research output: Contribution to journalArticle

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