The mechanism of membrane targeting of human sphingosine kinase 1

Robert V. Stahelin, Jeong H. Hwang, Jin Hahn Kim, Zee Yong Park, Korey R. Johnson, Lina M. Obeid, Wonhwa Cho

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Abstract

Sphingosine 1-phosphate is a bioactive sphingolipid that regulates cell growth and suppresses programmed cell death. The biosynthesis of sphingosine 1-phosphate is catalyzed by sphingosine kinase (SK) but the mechanism by which the subcellular localization and activity of SK is regulated in response to various stimuli is not fully understood. To elucidate the origin and structural determinant of the specific subcellular localization of SK, we performed biophysical and cell studies of human SK1 (hSK1) and selected mutants. In vitro measurements showed that hSK1 selectively bound phosphatidylserine over other anionic phospholipids and strongly preferred the plasma membrane-mimicking membrane to other cellular membrane mimetics. Mutational analysis indicates that conserved Thr54 and Asn89 in the putative membrane-binding surface are essential for lipid selectivity and membrane targeting both in vitro and in the cell. Also, phosphorylation of Ser 225 enhances the membrane affinity and plasma membrane selectivity of hSK1, presumably by modulating the interaction of Thr54 and Asn 89 with the membrane. Collectively, these studies suggest that the specific plasma membrane localization and activation of SK1 is mediated largely by specific lipid-protein interactions.

Original languageEnglish (US)
Pages (from-to)43030-43038
Number of pages9
JournalJournal of Biological Chemistry
Volume280
Issue number52
DOIs
StatePublished - Dec 30 2005

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Membranes
Cell membranes
Cell Membrane
Sphingolipids
Phosphorylation
Phosphatidylserines
Biosynthesis
Cell growth
Cell death
Membrane Lipids
Phospholipids
Cell Death
Chemical activation
sphingosine kinase
Lipids
Growth
Proteins
sphingosine 1-phosphate
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Stahelin, R. V., Hwang, J. H., Kim, J. H., Park, Z. Y., Johnson, K. R., Obeid, L. M., & Cho, W. (2005). The mechanism of membrane targeting of human sphingosine kinase 1. Journal of Biological Chemistry, 280(52), 43030-43038. https://doi.org/10.1074/jbc.M507574200

The mechanism of membrane targeting of human sphingosine kinase 1. / Stahelin, Robert V.; Hwang, Jeong H.; Kim, Jin Hahn; Park, Zee Yong; Johnson, Korey R.; Obeid, Lina M.; Cho, Wonhwa.

In: Journal of Biological Chemistry, Vol. 280, No. 52, 30.12.2005, p. 43030-43038.

Research output: Contribution to journalArticle

Stahelin, RV, Hwang, JH, Kim, JH, Park, ZY, Johnson, KR, Obeid, LM & Cho, W 2005, 'The mechanism of membrane targeting of human sphingosine kinase 1', Journal of Biological Chemistry, vol. 280, no. 52, pp. 43030-43038. https://doi.org/10.1074/jbc.M507574200
Stahelin, Robert V. ; Hwang, Jeong H. ; Kim, Jin Hahn ; Park, Zee Yong ; Johnson, Korey R. ; Obeid, Lina M. ; Cho, Wonhwa. / The mechanism of membrane targeting of human sphingosine kinase 1. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 52. pp. 43030-43038.
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