The medium chains of the mammalian clathrin-associated proteins have a homolog in yeast

Y. Nakayama, Mark Goebl, B. O'Brine Greco, S. Lemmon, E. Pingchang Chow, T. Kirchhausen

Research output: Contribution to journalArticle

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Abstract

We have cloned and sequenced mouse brain AP47, the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The predicted protein sequence of AP47 is closely related to rat and calf brain AP50, the corresponding medium chain of the plasma-membrane clathrin-associated protein complex AP-2. We have also identified in the yeast genome an open reading frame encoding a protein of previously unknown function. Referred to here as YAP54, its predicted protein sequence displays a striking homology to AP47. We therefore propose that Yap54 is the medium chain subunit of a putative AP-1 complex in yeast. From the analyses of the optimized sequence alignments of AP47, AP50 and Yap54p, we suggest a model for the domain organization of the medium chains.

Original languageEnglish (US)
Pages (from-to)569-574
Number of pages6
JournalEuropean Journal of Biochemistry
Volume202
Issue number2
DOIs
StatePublished - 1991

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Vesicular Transport Adaptor Proteins
Yeast
Yeasts
Transcription Factor AP-1
Brain
trans-Golgi Network
Proteins
Sequence Alignment
Cell membranes
Open Reading Frames
Rats
Membrane Proteins
Genes
Cell Membrane
Genome

ASJC Scopus subject areas

  • Biochemistry

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The medium chains of the mammalian clathrin-associated proteins have a homolog in yeast. / Nakayama, Y.; Goebl, Mark; O'Brine Greco, B.; Lemmon, S.; Pingchang Chow, E.; Kirchhausen, T.

In: European Journal of Biochemistry, Vol. 202, No. 2, 1991, p. 569-574.

Research output: Contribution to journalArticle

Nakayama, Y. ; Goebl, Mark ; O'Brine Greco, B. ; Lemmon, S. ; Pingchang Chow, E. ; Kirchhausen, T. / The medium chains of the mammalian clathrin-associated proteins have a homolog in yeast. In: European Journal of Biochemistry. 1991 ; Vol. 202, No. 2. pp. 569-574.
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AU - Kirchhausen, T.

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