The medium chains of the mammalian clathrin‐associated proteins have a homolog in yeast

Yasuhiro NAKAYAMA, Mark GOEBL, Betsy O'BRINE GRECO, Sandy LEMMON, Elizabeth PINGCHANG CHOW, Tomas KIRCHHAUSEN

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

We have cloned and sequenced mouse brain AP47, the medium chain of the trans‐Golgi network clathrin‐associated protein complex AP‐1. The predicted protein sequence of AP47 is closely related to rat and calf brain AP50, the corresponding medium chain of the plasma‐membrane clathrin‐associated protein complex AP‐2. We have also identified in the yeast genome an open reading frame encoding a protein of previously unknown function. Referred to here as YAP54, its predicted protein sequence displays a striking homology to AP47. We therefore propose that Yap54 is the medium chain subunit of a putative AP‐1 complex in yeast. From the analyses of the optimized sequence alignments of AP47, AP50 and Yap54p, we suggest a model for the domain organization of the medium chains.

Original languageEnglish (US)
Pages (from-to)569-574
Number of pages6
JournalEuropean Journal of Biochemistry
Volume202
Issue number2
DOIs
StatePublished - Dec 1991

ASJC Scopus subject areas

  • Biochemistry

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