the metabolism of l-asparagine in asparagus officinalis

David A. Cooney, Hiremagalur N. Jayaram, Stephen G. Swengros, Stephen C. Alter, Michael Levine

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

1. 1. l-Asparagine is present at a concentration ranging from 0.004-0.04 M in Asparagus officinalis. 2. 2. Sensitive radiometric techniques have been used to examine enzymes responsible for the synthesis and metabolism of l-asparagine in Asparagus. 3. 3. l-Asparagine synthetase was undetectable but p-cyano-L-alanine synthase was abundant. 4. 4. Additionally, β-cyano-l-alanine hydratase, several l-asparagine transaminases and a feeble, possibly exogenous, l-asparaginase were also detectable. 5. 5. Attempts to measure the substrates of β-cyano-l-alanine synthase in freeze-clamped specimens indicated that both l-eysteine and cyanide were present at concentrations well below the Km of the enzyme for them. 6. 6. Studies to demonstrate alternate routes for the biosynthesis of l-asparagine failed; thus, β-cyano-l-alanine synthase remains the most plausible candidate as the primary participant in the biosynthesis of l-asparagine in Asparagus.

Original languageEnglish (US)
Pages (from-to)69-83
Number of pages15
JournalInternational Journal of Biochemistry
Volume11
Issue number1
DOIs
StatePublished - 1980

ASJC Scopus subject areas

  • Biochemistry

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    Cooney, D. A., N. Jayaram, H., Swengros, S. G., Alter, S. C., & Levine, M. (1980). the metabolism of l-asparagine in asparagus officinalis. International Journal of Biochemistry, 11(1), 69-83. https://doi.org/10.1016/0020-711X(80)90281-5