the metabolism of l-asparagine in asparagus officinalis

David A. Cooney, Hiremagalur N. Jayaram, Stephen G. Swengros, Stephen C. Alter, Michael Levine

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

1. 1. l-Asparagine is present at a concentration ranging from 0.004-0.04 M in Asparagus officinalis. 2. 2. Sensitive radiometric techniques have been used to examine enzymes responsible for the synthesis and metabolism of l-asparagine in Asparagus. 3. 3. l-Asparagine synthetase was undetectable but p-cyano-L-alanine synthase was abundant. 4. 4. Additionally, β-cyano-l-alanine hydratase, several l-asparagine transaminases and a feeble, possibly exogenous, l-asparaginase were also detectable. 5. 5. Attempts to measure the substrates of β-cyano-l-alanine synthase in freeze-clamped specimens indicated that both l-eysteine and cyanide were present at concentrations well below the Km of the enzyme for them. 6. 6. Studies to demonstrate alternate routes for the biosynthesis of l-asparagine failed; thus, β-cyano-l-alanine synthase remains the most plausible candidate as the primary participant in the biosynthesis of l-asparagine in Asparagus.

Original languageEnglish (US)
Pages (from-to)69-83
Number of pages15
JournalInternational Journal of Biochemistry
Volume11
Issue number1
DOIs
StatePublished - 1980
Externally publishedYes

Fingerprint

Asparagus Plant
Asparagine
Metabolism
Alanine
asparagine-oxo-acid aminotransferase
Biosynthesis
Aspartate-Ammonia Ligase
Hydro-Lyases
Asparaginase
Cyanides
Enzymes
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cooney, D. A., N. Jayaram, H., Swengros, S. G., Alter, S. C., & Levine, M. (1980). the metabolism of l-asparagine in asparagus officinalis. International Journal of Biochemistry, 11(1), 69-83. https://doi.org/10.1016/0020-711X(80)90281-5

the metabolism of l-asparagine in asparagus officinalis. / Cooney, David A.; N. Jayaram, Hiremagalur; Swengros, Stephen G.; Alter, Stephen C.; Levine, Michael.

In: International Journal of Biochemistry, Vol. 11, No. 1, 1980, p. 69-83.

Research output: Contribution to journalArticle

Cooney, DA, N. Jayaram, H, Swengros, SG, Alter, SC & Levine, M 1980, 'the metabolism of l-asparagine in asparagus officinalis', International Journal of Biochemistry, vol. 11, no. 1, pp. 69-83. https://doi.org/10.1016/0020-711X(80)90281-5
Cooney, David A. ; N. Jayaram, Hiremagalur ; Swengros, Stephen G. ; Alter, Stephen C. ; Levine, Michael. / the metabolism of l-asparagine in asparagus officinalis. In: International Journal of Biochemistry. 1980 ; Vol. 11, No. 1. pp. 69-83.
@article{0e68974c7fb245edba13b12d8d62578f,
title = "the metabolism of l-asparagine in asparagus officinalis",
abstract = "1. 1. l-Asparagine is present at a concentration ranging from 0.004-0.04 M in Asparagus officinalis. 2. 2. Sensitive radiometric techniques have been used to examine enzymes responsible for the synthesis and metabolism of l-asparagine in Asparagus. 3. 3. l-Asparagine synthetase was undetectable but p-cyano-L-alanine synthase was abundant. 4. 4. Additionally, β-cyano-l-alanine hydratase, several l-asparagine transaminases and a feeble, possibly exogenous, l-asparaginase were also detectable. 5. 5. Attempts to measure the substrates of β-cyano-l-alanine synthase in freeze-clamped specimens indicated that both l-eysteine and cyanide were present at concentrations well below the Km of the enzyme for them. 6. 6. Studies to demonstrate alternate routes for the biosynthesis of l-asparagine failed; thus, β-cyano-l-alanine synthase remains the most plausible candidate as the primary participant in the biosynthesis of l-asparagine in Asparagus.",
author = "Cooney, {David A.} and {N. Jayaram}, Hiremagalur and Swengros, {Stephen G.} and Alter, {Stephen C.} and Michael Levine",
year = "1980",
doi = "10.1016/0020-711X(80)90281-5",
language = "English (US)",
volume = "11",
pages = "69--83",
journal = "International Journal of Biochemistry and Cell Biology",
issn = "1357-2725",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - the metabolism of l-asparagine in asparagus officinalis

AU - Cooney, David A.

AU - N. Jayaram, Hiremagalur

AU - Swengros, Stephen G.

AU - Alter, Stephen C.

AU - Levine, Michael

PY - 1980

Y1 - 1980

N2 - 1. 1. l-Asparagine is present at a concentration ranging from 0.004-0.04 M in Asparagus officinalis. 2. 2. Sensitive radiometric techniques have been used to examine enzymes responsible for the synthesis and metabolism of l-asparagine in Asparagus. 3. 3. l-Asparagine synthetase was undetectable but p-cyano-L-alanine synthase was abundant. 4. 4. Additionally, β-cyano-l-alanine hydratase, several l-asparagine transaminases and a feeble, possibly exogenous, l-asparaginase were also detectable. 5. 5. Attempts to measure the substrates of β-cyano-l-alanine synthase in freeze-clamped specimens indicated that both l-eysteine and cyanide were present at concentrations well below the Km of the enzyme for them. 6. 6. Studies to demonstrate alternate routes for the biosynthesis of l-asparagine failed; thus, β-cyano-l-alanine synthase remains the most plausible candidate as the primary participant in the biosynthesis of l-asparagine in Asparagus.

AB - 1. 1. l-Asparagine is present at a concentration ranging from 0.004-0.04 M in Asparagus officinalis. 2. 2. Sensitive radiometric techniques have been used to examine enzymes responsible for the synthesis and metabolism of l-asparagine in Asparagus. 3. 3. l-Asparagine synthetase was undetectable but p-cyano-L-alanine synthase was abundant. 4. 4. Additionally, β-cyano-l-alanine hydratase, several l-asparagine transaminases and a feeble, possibly exogenous, l-asparaginase were also detectable. 5. 5. Attempts to measure the substrates of β-cyano-l-alanine synthase in freeze-clamped specimens indicated that both l-eysteine and cyanide were present at concentrations well below the Km of the enzyme for them. 6. 6. Studies to demonstrate alternate routes for the biosynthesis of l-asparagine failed; thus, β-cyano-l-alanine synthase remains the most plausible candidate as the primary participant in the biosynthesis of l-asparagine in Asparagus.

UR - http://www.scopus.com/inward/record.url?scp=0018873907&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018873907&partnerID=8YFLogxK

U2 - 10.1016/0020-711X(80)90281-5

DO - 10.1016/0020-711X(80)90281-5

M3 - Article

VL - 11

SP - 69

EP - 83

JO - International Journal of Biochemistry and Cell Biology

JF - International Journal of Biochemistry and Cell Biology

SN - 1357-2725

IS - 1

ER -