The metabolism of vitamin D3 and 25 hydroxyvitamin D3 in normal and anephric humans

R. W. Gray, H. P. Weber, J. H. Dominguez, J. Lemann

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

To clarify in human beings the further metabolism of 25 OH D3, the principal circulating form of vitamin D, the fates of 26,27 3H 25 OH D3 or 1,2 3H 4 14C D3 were compared in 6 normal subjects and 10 anephric patients on chronic hemodialysis. Normal subjects and 6 of 10 anephrics formed 24,25 (OH)2 D3 suggesting that the kidney may not be the only tissue capable of 24 hydroxylation. Normal subjects also produced metabolites comigrating with authentic 1,25 (OH)2 D3 and 1,24 25 (OH)3 D3. Anephrics did not produce metabolites comigrating with 1,25 (OH)2 D3 but their serum did contain small amounts of metabolites comigrating with authentic 1,24,25 (OH)3 D3. Estimated plasma 25 OH D3 pools were indistinguishable in the 2 groups averaging 91±9 μg in normals and 96±8 μg in anephrics. However, the estimated turnover rate of 25 OH D3 in anephrics was only 1.6±0.1 μg/day as compared to 2.8±0.3 μg/day in normals (p3 more polar then 1,24,25 (OH)3 D3 were also observed in plasma, urine, bile, and feces of both normals and anephrics. Their identity and biologic significance remain to be determined.

Original languageEnglish (US)
Pages (from-to)1045-1056
Number of pages12
JournalJournal of Clinical Endocrinology and Metabolism
Volume39
Issue number6
StatePublished - 1974
Externally publishedYes

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Calcifediol
Cholecalciferol
Metabolites
Metabolism
Hydroxylation
Feces
Bile
Vitamin D
Plasmas
Renal Dialysis
Urine
Kidney
Serum
Tissue

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

The metabolism of vitamin D3 and 25 hydroxyvitamin D3 in normal and anephric humans. / Gray, R. W.; Weber, H. P.; Dominguez, J. H.; Lemann, J.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 39, No. 6, 1974, p. 1045-1056.

Research output: Contribution to journalArticle

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