The methyltransferase YfgB/RlmN is responsible for modification of adenosine 2503 in 23S rRNA

Seok Ming Toh, Liqun Xiong, Taeok Bae, Alexander S. Mankin

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

A2503 in 23S rRNA of the Gram-negative bacterium Escherichia coli is located in a functionally important region of the ribosome, at the entrance to the nascent peptide exit tunnel. In E. coli, and likely in other species, this adenosine residue is posttranscriptionally modified to m2A. The enzyme responsible for this modification was previously unknown. We identified E. coli protein YfgB, which belongs to the radical SAM enzyme superfamily, as the methyltransferase that modifies A2503 of 23S rRNA to m2A. Inactivation of the yfgB gene in E. coli led to the loss of modification at nucleotide A2503 of 23S rRNA as revealed by primer extension analysis and thin layer chromatography. The A2503 modification was restored when YfgB protein was expressed in the yfgB knockout strain. A similar protein was shown to catalyze post-transcriptional modification of A2503 in 23S rRNA in Gram-positive Staphylococcus aureus. The yfgB knockout strain loses in competition with wild type in a co-growth experiment, indicating functional importance of A2503 modification. The location of A2503 in the exit tunnel suggests its possible involvement in interaction with the nascent peptide and raises the possibility that its post-transcriptional modification may influence such an interaction. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish
Pages (from-to)98-106
Number of pages9
JournalRNA
Volume14
Issue number1
DOIs
StatePublished - Jan 2008

Fingerprint

Methyltransferases
Adenosine
Escherichia coli
Peptides
Escherichia coli Proteins
Gene Silencing
Enzymes
Thin Layer Chromatography
Gram-Negative Bacteria
Ribosomes
Staphylococcus aureus
Proteins
Nucleotides
Growth

Keywords

  • Exit tunnel
  • Peptidyl transferase
  • Post-transcriptional modifications
  • Radical SAM methyl transferase
  • Ribosome

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

The methyltransferase YfgB/RlmN is responsible for modification of adenosine 2503 in 23S rRNA. / Toh, Seok Ming; Xiong, Liqun; Bae, Taeok; Mankin, Alexander S.

In: RNA, Vol. 14, No. 1, 01.2008, p. 98-106.

Research output: Contribution to journalArticle

Toh, Seok Ming ; Xiong, Liqun ; Bae, Taeok ; Mankin, Alexander S. / The methyltransferase YfgB/RlmN is responsible for modification of adenosine 2503 in 23S rRNA. In: RNA. 2008 ; Vol. 14, No. 1. pp. 98-106.
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