The microbial communities in male first catch urine are highly similar to those in paired urethral swab specimens

Qunfeng Dong, David E. Nelson, Evelyn Toh, Lixia Diao, Xiang Gao, J. Fortenberry, Barbara van Der Pol

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Urine is the CDC-recommended specimen for STI testing. It was unknown if the bacterial communities (microbiomes) in urine reflected those in the distal male urethra. We compared microbiomes of 32 paired urine and urethral swab specimens obtained from adult men attending an STD clinic, by 16S rRNA PCR and deep pyrosequencing. Microbiomes of urine and swabs were remarkably similar, regardless of STI status of the subjects. Thus, urine can be used to characterize urethral microbiomes when swabs are undesirable, such as in population-based studies of the urethral microbiome or where multiple sampling of participants is required.

Original languageEnglish
Article numbere19709
JournalPLoS One
Volume6
Issue number5
DOIs
StatePublished - 2011

Fingerprint

Microbiota
microbial communities
urine
Urine
Sampling
Sexually Transmitted Diseases
Testing
urethra
Urethra
Centers for Disease Control and Prevention (U.S.)
bacterial communities
microbiome
ribosomal RNA
Polymerase Chain Reaction
Population
testing
sampling

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

The microbial communities in male first catch urine are highly similar to those in paired urethral swab specimens. / Dong, Qunfeng; Nelson, David E.; Toh, Evelyn; Diao, Lixia; Gao, Xiang; Fortenberry, J.; van Der Pol, Barbara.

In: PLoS One, Vol. 6, No. 5, e19709, 2011.

Research output: Contribution to journalArticle

Dong, Qunfeng ; Nelson, David E. ; Toh, Evelyn ; Diao, Lixia ; Gao, Xiang ; Fortenberry, J. ; van Der Pol, Barbara. / The microbial communities in male first catch urine are highly similar to those in paired urethral swab specimens. In: PLoS One. 2011 ; Vol. 6, No. 5.
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