The microtubule-destabilizing kinesin XKCM1 is required for chromosome positioning during spindle assembly

Claire Walczak, Eugene C. Gan, Arshad Desai, Timothy J. Mitchison, Susan L. Kline-Smith

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Xenopus kinesin catastrophe modulator-1 (XKCM1) is a Kin I kinesin family member that uses the energy of ATP hydrolysis to depolymerize microtubules [1-3]. We demonstrated previously that XKCM1 is essential for mitotic-spindle assembly in vitro and acts by regulating microtubule dynamics as a pure protein, in extracts and in cells [2, 4, 5]. A portion of the XKCM1 pool is specifically localized to centromeres during mitosis and may be important in chromosome movement. To selectively analyze the function of centromere-bound XKCM1, we generated glutathione-S-transferase (GST) fusion proteins containing the N-terminal globular domain (GST-NT), the centrally located catalytic domain (GST-CD), and the C-terminal α-helical tail (GST-CT) of XKCM1. The GST-NT protein targeted to centromeres during spindle assembly, suggesting that the N-terminal domain of XKCM1 is sufficient for centromere localization. Addition of GST-NT prior to or after spindle assembly replaced endogenous XKCM1, indicating that centromere targeting is a dynamic process. Loss of endogenous XKCM1 from centromeres caused a misalignment of chromosomes on the metaphase plate without affecting global spindle structure. These results suggest that centromere bound XKCM1 has an important role in chromosome positioning on the spindle.

Original languageEnglish
Pages (from-to)1885-1889
Number of pages5
JournalCurrent Biology
Volume12
Issue number21
DOIs
StatePublished - Oct 29 2002

Fingerprint

Chromosome Positioning
Kinesin
kinesin
Xenopus
Chromosomes
Microtubules
Modulators
microtubules
Centromere
centromeres
chromosomes
Glutathione Transferase
glutathione transferase
mitotic spindle apparatus
Spindle Apparatus
Proteins
proteins
Metaphase
Cell Extracts
metaphase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

The microtubule-destabilizing kinesin XKCM1 is required for chromosome positioning during spindle assembly. / Walczak, Claire; Gan, Eugene C.; Desai, Arshad; Mitchison, Timothy J.; Kline-Smith, Susan L.

In: Current Biology, Vol. 12, No. 21, 29.10.2002, p. 1885-1889.

Research output: Contribution to journalArticle

Walczak, Claire ; Gan, Eugene C. ; Desai, Arshad ; Mitchison, Timothy J. ; Kline-Smith, Susan L. / The microtubule-destabilizing kinesin XKCM1 is required for chromosome positioning during spindle assembly. In: Current Biology. 2002 ; Vol. 12, No. 21. pp. 1885-1889.
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