The Mitochondrial Aldehyde Dehydrogenase Gene Resides in an HTF Island but Is Expressed in a Tissue-Specific Manner

K. M. Dipple, David Crabb

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The tissue distribution of mitochondrial aldehyde dehydrogenase (ALDH2) in rats was analyzed by activity assays, and by Western and Northern blotting. ALDH2 was expressed at highest levels in liver. The mRNA levels were intermediate in the kidney and lung, while lower levels were found in spleen and heart. The transcript was undetectable in other tissues tested. The human ALDH2 5′ flanking region (-200 to +60) contains similar numbers of CpG and GpC dinucleotides and the rat ALDH2 gene was undermethylated in liver, kidney, and spleen. This suggests that the ALDH2 promoter resides in a Hpa II tiny fragment (HTF) island, unlike most genes expressed in a tissue-specific manner.

Original languageEnglish
Pages (from-to)420-427
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume193
Issue number1
DOIs
StatePublished - May 28 1993

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Aldehyde Dehydrogenase
Islands
Spleen
Genes
Tissue
Kidney
Liver
Rats
5' Flanking Region
Tissue Distribution
Northern Blotting
Western Blotting
Lung
Messenger RNA
Assays
Mitochondrial Aldehyde Dehydrogenase

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

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