The monoclonal antibody CZ-1 identifies a mouse CD45-associated epitope expressed on interleukin-2-responsive cells

Randy Brutkiewicz, Carey L. O'Donnell, Joseph W. Maciaszek, Raymond M. Welsh, Mauricio Vargas-Cortes

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have previously described a monoclonal antibody (mAb), CZ-1, which reacts with an epitope expressed on most peripheral basophils, natural killer cells, B cells, and CD8+ T cells, but not with most thymocytes or peripheral CD4+ T cells. Here we show that mAb CZ-1 defines a sialic acid-dependent epitope associated with a subpopulation of CD45 molecules. This conclusion is based on the ability to block binding of mAb CZ-1 by sialic acid, neuramin-lactose, neuraminidase, and mAb to CD45RB, and by expression of the epitope on transfected ψ2 cells expressing exon B of CD45. The results suggest that the CZ-1 epitope is a post-translational modification expressed on a subpopulation of the CD45 molecules also expressing the B exon. Expression of the CZ-1 epitope was required for freshly isolated lymphocytes to respond to interleukin-2 (IL-2). Depletion of CZ-1+ cells by C′ or by cell sorting of thymocytes or splenocytes eliminated the IL-2 responsive cells.The subpopulations of thymocytes and CD4+ splenocytes responding to L-2 were exclusively within the small CZ-1+ subpopulation. mAb CZ-1 was also used to subdivide CD45+ and CD45RB+ splenocytes into IL-2-responsive and -nonresponsive subpopulations. The CZ-1 epitope was also expressed on virtually all lymphokine-activated killer cell precursors. These data, thus, indicate that cells responsive to IL-2 express this sialated modification of CD45.

Original languageEnglish (US)
Pages (from-to)2427-2433
Number of pages7
JournalEuropean Journal of Immunology
Volume23
Issue number10
StatePublished - Oct 1993
Externally publishedYes

Fingerprint

Interleukin-2
Epitopes
Monoclonal Antibodies
Thymocytes
N-Acetylneuraminic Acid
Exons
T-Lymphocytes
Lymphokine-Activated Killer Cells
Basophils
Neuraminidase
Post Translational Protein Processing
Lactose
Natural Killer Cells
B-Lymphocytes
Lymphocytes

Keywords

  • CD45
  • Interleukin-2
  • Monoclonal antibody CZ-1
  • Tlymphocyte

ASJC Scopus subject areas

  • Immunology

Cite this

The monoclonal antibody CZ-1 identifies a mouse CD45-associated epitope expressed on interleukin-2-responsive cells. / Brutkiewicz, Randy; O'Donnell, Carey L.; Maciaszek, Joseph W.; Welsh, Raymond M.; Vargas-Cortes, Mauricio.

In: European Journal of Immunology, Vol. 23, No. 10, 10.1993, p. 2427-2433.

Research output: Contribution to journalArticle

Brutkiewicz, Randy ; O'Donnell, Carey L. ; Maciaszek, Joseph W. ; Welsh, Raymond M. ; Vargas-Cortes, Mauricio. / The monoclonal antibody CZ-1 identifies a mouse CD45-associated epitope expressed on interleukin-2-responsive cells. In: European Journal of Immunology. 1993 ; Vol. 23, No. 10. pp. 2427-2433.
@article{120c4eaaa20d4697a2b42ae7d0b02940,
title = "The monoclonal antibody CZ-1 identifies a mouse CD45-associated epitope expressed on interleukin-2-responsive cells",
abstract = "We have previously described a monoclonal antibody (mAb), CZ-1, which reacts with an epitope expressed on most peripheral basophils, natural killer cells, B cells, and CD8+ T cells, but not with most thymocytes or peripheral CD4+ T cells. Here we show that mAb CZ-1 defines a sialic acid-dependent epitope associated with a subpopulation of CD45 molecules. This conclusion is based on the ability to block binding of mAb CZ-1 by sialic acid, neuramin-lactose, neuraminidase, and mAb to CD45RB, and by expression of the epitope on transfected ψ2 cells expressing exon B of CD45. The results suggest that the CZ-1 epitope is a post-translational modification expressed on a subpopulation of the CD45 molecules also expressing the B exon. Expression of the CZ-1 epitope was required for freshly isolated lymphocytes to respond to interleukin-2 (IL-2). Depletion of CZ-1+ cells by C′ or by cell sorting of thymocytes or splenocytes eliminated the IL-2 responsive cells.The subpopulations of thymocytes and CD4+ splenocytes responding to L-2 were exclusively within the small CZ-1+ subpopulation. mAb CZ-1 was also used to subdivide CD45+ and CD45RB+ splenocytes into IL-2-responsive and -nonresponsive subpopulations. The CZ-1 epitope was also expressed on virtually all lymphokine-activated killer cell precursors. These data, thus, indicate that cells responsive to IL-2 express this sialated modification of CD45.",
keywords = "CD45, Interleukin-2, Monoclonal antibody CZ-1, Tlymphocyte",
author = "Randy Brutkiewicz and O'Donnell, {Carey L.} and Maciaszek, {Joseph W.} and Welsh, {Raymond M.} and Mauricio Vargas-Cortes",
year = "1993",
month = "10",
language = "English (US)",
volume = "23",
pages = "2427--2433",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "10",

}

TY - JOUR

T1 - The monoclonal antibody CZ-1 identifies a mouse CD45-associated epitope expressed on interleukin-2-responsive cells

AU - Brutkiewicz, Randy

AU - O'Donnell, Carey L.

AU - Maciaszek, Joseph W.

AU - Welsh, Raymond M.

AU - Vargas-Cortes, Mauricio

PY - 1993/10

Y1 - 1993/10

N2 - We have previously described a monoclonal antibody (mAb), CZ-1, which reacts with an epitope expressed on most peripheral basophils, natural killer cells, B cells, and CD8+ T cells, but not with most thymocytes or peripheral CD4+ T cells. Here we show that mAb CZ-1 defines a sialic acid-dependent epitope associated with a subpopulation of CD45 molecules. This conclusion is based on the ability to block binding of mAb CZ-1 by sialic acid, neuramin-lactose, neuraminidase, and mAb to CD45RB, and by expression of the epitope on transfected ψ2 cells expressing exon B of CD45. The results suggest that the CZ-1 epitope is a post-translational modification expressed on a subpopulation of the CD45 molecules also expressing the B exon. Expression of the CZ-1 epitope was required for freshly isolated lymphocytes to respond to interleukin-2 (IL-2). Depletion of CZ-1+ cells by C′ or by cell sorting of thymocytes or splenocytes eliminated the IL-2 responsive cells.The subpopulations of thymocytes and CD4+ splenocytes responding to L-2 were exclusively within the small CZ-1+ subpopulation. mAb CZ-1 was also used to subdivide CD45+ and CD45RB+ splenocytes into IL-2-responsive and -nonresponsive subpopulations. The CZ-1 epitope was also expressed on virtually all lymphokine-activated killer cell precursors. These data, thus, indicate that cells responsive to IL-2 express this sialated modification of CD45.

AB - We have previously described a monoclonal antibody (mAb), CZ-1, which reacts with an epitope expressed on most peripheral basophils, natural killer cells, B cells, and CD8+ T cells, but not with most thymocytes or peripheral CD4+ T cells. Here we show that mAb CZ-1 defines a sialic acid-dependent epitope associated with a subpopulation of CD45 molecules. This conclusion is based on the ability to block binding of mAb CZ-1 by sialic acid, neuramin-lactose, neuraminidase, and mAb to CD45RB, and by expression of the epitope on transfected ψ2 cells expressing exon B of CD45. The results suggest that the CZ-1 epitope is a post-translational modification expressed on a subpopulation of the CD45 molecules also expressing the B exon. Expression of the CZ-1 epitope was required for freshly isolated lymphocytes to respond to interleukin-2 (IL-2). Depletion of CZ-1+ cells by C′ or by cell sorting of thymocytes or splenocytes eliminated the IL-2 responsive cells.The subpopulations of thymocytes and CD4+ splenocytes responding to L-2 were exclusively within the small CZ-1+ subpopulation. mAb CZ-1 was also used to subdivide CD45+ and CD45RB+ splenocytes into IL-2-responsive and -nonresponsive subpopulations. The CZ-1 epitope was also expressed on virtually all lymphokine-activated killer cell precursors. These data, thus, indicate that cells responsive to IL-2 express this sialated modification of CD45.

KW - CD45

KW - Interleukin-2

KW - Monoclonal antibody CZ-1

KW - Tlymphocyte

UR - http://www.scopus.com/inward/record.url?scp=0027489998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027489998&partnerID=8YFLogxK

M3 - Article

C2 - 7691607

AN - SCOPUS:0027489998

VL - 23

SP - 2427

EP - 2433

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 10

ER -