The morphology of the renal microvasculature in glycerol- and gentamicin-induced acute renal failure

Vincent H. Gattone, Andrew P. Evan, Stephen A. Mong, Bret A. Connors, George R. Aronoff, Friedrich C. Luft

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11 Scopus citations

Abstract

To elucidate abnormalities in the renal microvasculature that could account for the functional disturbances occurring in two well-established models of acute renal failure, we gave rats a single intramuscular injection of glycerol (50%, 10 ml/kg) or daily subcutaneous doses of gentamicin (100 mg/kg/day). Afferent arteriolar diameters were determined by measuring methacrylate vascular casts with SEM. The filtration barrier was examined by both SEM and TEM. The EF area was quantitated. By 3 hr, the glycerol treatment markedly decreased PADs and DADs (PAD 19.1 to 12.0 μm, DAD 13.8 to 7.4 μm, p < 0.05). The changes were similar for both inner and outer cortical regions. By 3 days the vasoconstriction was alleviated; however, renal failure persisted. At that time, however, EF area was decreased to 43% of normal. After 10 days of gentamicin treatment, only minimal vasoconstriction occurred in the outer cortex; however, EF area was decreased to a similar degree as observed with the 3-day glycerol-treated animals. There are two phases to glycerol-induced acute renal failure. The first phase (described as readily reversible) is characterized by intense vasoconstriction. The second phase, which is not immediately reversible, is associated with a decreased EF area. Smaller outer cortical afferent arterioles and a decreased fenestral diameter and density of the glomerular endothelium are seen only after gentamicin-induced renal failure is well established (after 10 days of treatment).

Original languageEnglish (US)
Pages (from-to)183-195
Number of pages13
JournalThe Journal of Laboratory and Clinical Medicine
Volume101
Issue number2
StatePublished - Feb 1983

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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