The n terminus of pro-endothelial monocyte-activating polypeptide ii (emap ii) regulates its binding with the C terminus, Arginyl-trna synthetase, and neurofilament light protein

Haiming Xu, Nikolay L. Malinin, Niranjan Awasthi, Roderich E. Schwarz, Margaret Schwarz

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Pro-endothelial monocyte-activating polypeptide II (EMAP II), one component of the multi-aminoacyl tRNA synthetase complex, plays multiple roles in physiological and pathological processes of protein translation, signal transduction, immunity, lung development, and tumor growth. Recent studies have determined that pro-EMAP II has an essential role in maintaining axon integrity in central and peripheral neural systems where deletion of the C terminus of pro-EMAP II has been reported in a consanguineous Israeli Bedouin kindred suffering from Pelizaeus-Merzbacher-like disease. We hypothesized that the N terminus of pro-EMAP II has an important role in the regulation of protein-protein interactions. Using aGFPreporter system, we defined a putative leucine zipper in theNterminus of human pro-EMAP II protein (amino acid residues 1-70) that can form specific strip-like punctate structures. Through GFP punctum analysis, we uncovered that the pro-EMAP II C terminus (amino acids 147-312) can repress GFP punctum formation. Pulldown assays confirmed that the binding between the pro-EMAP II N terminus and its C terminus is mediated by a putative leucine zipper. Furthermore, the pro-EMAP II 1-70 amino acid region was identified as the binding partner of argi-nyl-tRNA synthetase, a polypeptide of the multi-aminoacyl tRNA synthetase complex. We also determined that the punctate GFP pro-EMAP II 1-70 amino acid aggregate colocalizes and binds to the neurofilament light subunit protein that is associated with pathologic neurofilament network disorganization and degeneration of motor neurons. These findings indicate the structure and binding interaction of pro-EMAP II protein and suggest a role of this protein in pathological neurodegenerative diseases.

Original languageEnglish
Pages (from-to)9753-9766
Number of pages14
JournalJournal of Biological Chemistry
Volume290
Issue number15
DOIs
StatePublished - Apr 10 2015

Fingerprint

Ligases
Monocytes
Peptides
Amino Acyl-tRNA Synthetases
Amino Acids
Leucine Zippers
Proteins
Pelizaeus-Merzbacher Disease
neurofilament protein L
small inducible cytokine subfamily E, member 1
Neurodegenerative diseases
Physiological Phenomena
Signal transduction
Intermediate Filaments
Protein Subunits
Protein Biosynthesis
Motor Neurons
Pathologic Processes
Growth and Development
Neurodegenerative Diseases

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

The n terminus of pro-endothelial monocyte-activating polypeptide ii (emap ii) regulates its binding with the C terminus, Arginyl-trna synthetase, and neurofilament light protein. / Xu, Haiming; Malinin, Nikolay L.; Awasthi, Niranjan; Schwarz, Roderich E.; Schwarz, Margaret.

In: Journal of Biological Chemistry, Vol. 290, No. 15, 10.04.2015, p. 9753-9766.

Research output: Contribution to journalArticle

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