The nematode C. elegans as an animal model to explore toxicology in vivo

solid and axenic growth culture conditions and compound exposure parameters.

Richard Nass, Iqbal Hamza

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Significant limitations in vertebrate animal model systems include the time involved, the expense, the fact that in vitro results may not reflect live animal pathology, difficulties in transporting the toxin past the blood brain barrier, and the inability to identify the mechanism of action without some a priori knowledge of the toxin's target. The availability of the complete genome sequence of the nematode C. elegans, coupled with the worm's size, growth rate, ease of culturing, and the realization that basic biological mechanisms and disease processes between worms and humans are highly conserved, makes this genetically tractable model a remarkable opportunity to dissect and identify in vivo the cellular processes involved in toxin-induced cell dysregulation and death. This unit includes protocols for culturing worms on solid and axenic media and acute and chronic exposure parameters for Parkinson's disease-associated toxins and hemin chloride. These methods provide the groundwork for using this powerful model system to further elucidate and understand the molecular mechanisms involved in nutrition as well as toxicological responses relevant to human diseases.

Original languageEnglish
JournalCurrent protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]
VolumeChapter 1
StatePublished - Feb 2007
Externally publishedYes

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Axenic Culture
Toxicology
Animals
Animal Models
Hemin
Growth
Blood-Brain Barrier
Parkinson Disease
Vertebrates
Chlorides
Cell Death
Genome
Pathology
Nutrition
Genes
Availability
In Vitro Techniques

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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