The Novel Class III Antiarrhythmics NE-10064 and NE-10133 Inhibit Isk Channels Expressed in Xenopus Oocytes and Iks in Guinea Pig Cardiac Myocytes

A. E. Busch, K. Malloy, William Groh, M. D. Varnum, J. P. Adelman, J. Maylie

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Slowly activating, voltage-dependent IsK channels were expressed in Xenopus oocytes after injection of rat IsK protein cRNA and recorded with the two-microelectrode voltage-clamp technique. The IsK currents were inhibited by the new class III antiarrhythmic drugs NE-10064 and NE-10133. These compounds were equally potent in inhibiting a slowly activating potassium current (IKs) in guinea pig ventricular myocytes. No effects of these compounds could be observed on several other cloned delayed rectifier potassium channels, nor did they affect the inward rectifier current, IK1, in guinea pig cardiac myocytes at the concentrations tested. The blockade of IsK channels may contribute to the class III antiarrhythmic efficacy of these novel antiarrhythmics.

Original languageEnglish (US)
Pages (from-to)265-270
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume202
Issue number1
DOIs
StatePublished - Jul 15 1994
Externally publishedYes

Fingerprint

Xenopus
Cardiac Myocytes
Oocytes
Delayed Rectifier Potassium Channels
Guinea Pigs
Complementary RNA
Microelectrodes
Anti-Arrhythmia Agents
Clamping devices
Electric potential
Patch-Clamp Techniques
Muscle Cells
Rats
Potassium
Injections
Proteins
NE 10133
azimilide

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

The Novel Class III Antiarrhythmics NE-10064 and NE-10133 Inhibit Isk Channels Expressed in Xenopus Oocytes and Iks in Guinea Pig Cardiac Myocytes. / Busch, A. E.; Malloy, K.; Groh, William; Varnum, M. D.; Adelman, J. P.; Maylie, J.

In: Biochemical and Biophysical Research Communications, Vol. 202, No. 1, 15.07.1994, p. 265-270.

Research output: Contribution to journalArticle

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