The nucleotide sequence of the ilvBN operon of Escherichia coli: Sequence homologies of the acetohydroxy acid synthase isozymes

Ronald Wek, Craig A. Hauser, G. Wesley Hatfield

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Three acetohydroxy acid synthase isozymes, AHAS I (ilvBN), AHAS II (ilvGM) and AHAS III (ilvIH) catalyze the first step of the parallel isoleucine-valine biosynthetic pathway in Escherichia coli. Previous DNA sequence and protein purification data have shown that AHAS II and AHAS III are composed of large and small subunits encoded in the ilvGMEDA and ilvIH operons, respectively. Recent protein purification and charac-terization data have demonstrated that the AHAS I isozyme is also composed of large and small subunits (L. Eoyang, L. and P. M. Silverman [1984] J. Bacteriol. 157:184-189). Now the complete DNA sequence of the operon encoding the AHAS I isozyme has been determined. These data show that both AHAS I subunits (Mr 60,400 and Mr. 11,100) are encoded in this operon. The coordinant regulation of both genes of the ilvBN operon has also been demonstrated. Comparisons of the DNA sequences of the genes encoding all three AHAS isozymes have been performed. Conserved homologies were observed between both the large and small subunits of all three isozymes. The closest homology was seen between the AHAS I and AHAS II isozymes. On the basis of these comparisons a rationale for the evolution of the AHAS isozymes in E. coli has been proposed

Original languageEnglish (US)
Pages (from-to)3995-4010
Number of pages16
JournalNucleic Acids Research
Volume13
Issue number11
DOIs
StatePublished - Jun 11 1985
Externally publishedYes

Fingerprint

Acetolactate Synthase
DNA sequences
Operon
Sequence Homology
Nucleotides
DNA Sequence
Escherichia coli
Escherichia Coli
Isoenzymes
Homology
Purification
Acids
Encoding
Gene
Proteins
Protein
Gene encoding
Pathway
Genes
Isoleucine

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

The nucleotide sequence of the ilvBN operon of Escherichia coli : Sequence homologies of the acetohydroxy acid synthase isozymes. / Wek, Ronald; Hauser, Craig A.; Hatfield, G. Wesley.

In: Nucleic Acids Research, Vol. 13, No. 11, 11.06.1985, p. 3995-4010.

Research output: Contribution to journalArticle

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