The origin of serotoninergic projections to the lumbosacral spinal cord at different stages of development in the North American opossum

G. F. Martin, G. Ghooray, R. H. Ho, R. R. Pindzola, Xiao-Ming Xu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have employed immunohistochemistry and the retrograde transport of Fast blue to study the origin of serotoninergic projections to the lumbosacral spinal cord at different stages of development in the North American opossum. A few serotoninergic axons are present in the lumbosacral cord at birth, 12 days after conception, and serotoninergic neurons are numerous in the brainstem where they are present in most, if not all, of the areas which contain them in the adult animals. A few neurons of the caudal raphe and adjacent reticular formation were labeled by lumbar injections of Fast blue on postnatal day 1, and by postnatal day 3, labeled neurons were numerous within all areas which provide serotoninergic projections to the lumbosacral cord in adult animals. By postnatal day 11, it was possible to combine Fast blue labeling with immunofluorescence to show that some of the labeled neurons were serotoninergic. By postnatal day 24, neurons which provide serotoninergic projections to the lumbosacral cord were especially numerous and some of them were found in areas which do not provide comparable projections in adult animals. In developing and adult animals, few, if any, neurons were labeled in the dorsal raphe or superior central nuclei. We have shown previously that serotoninergic axons do not innervate laminae I and II of the lumbosacral cord until approximately postnatal day 50, although they are present in the marginal zone at birth and have grown into laminae III-X by postnatal day 15. Since serotoninergic axons which project to laminae I and II originate within the raphe magnus and adjacent reticular formation, and those areas provide serotoninergic projections to the spinal cord well before postnatal day 50, it is possible that serotoninergic innervation of laminae I and II is provided by late growth of collaterals from axons that have been present in the marginal zone for some time.

Original languageEnglish (US)
Pages (from-to)203-213
Number of pages11
JournalDevelopmental Brain Research
Volume58
Issue number2
DOIs
StatePublished - Feb 22 1991
Externally publishedYes

Fingerprint

Didelphis
Substantia Gelatinosa
Axons
Spinal Cord
Serotonergic Neurons
Neurons
Reticular Formation
Parturition
Raphe Nuclei
Brain Stem
Fluorescent Antibody Technique
Immunohistochemistry
Injections
Spinal Cord Dorsal Horn
Growth
diamidino compound 253-50

Keywords

  • Development
  • Opossum
  • Raphe nucleus
  • Reticular formation
  • Serotonin
  • Spinal cord

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

The origin of serotoninergic projections to the lumbosacral spinal cord at different stages of development in the North American opossum. / Martin, G. F.; Ghooray, G.; Ho, R. H.; Pindzola, R. R.; Xu, Xiao-Ming.

In: Developmental Brain Research, Vol. 58, No. 2, 22.02.1991, p. 203-213.

Research output: Contribution to journalArticle

@article{a998ad19fa70431d97fec46c85ca4683,
title = "The origin of serotoninergic projections to the lumbosacral spinal cord at different stages of development in the North American opossum",
abstract = "We have employed immunohistochemistry and the retrograde transport of Fast blue to study the origin of serotoninergic projections to the lumbosacral spinal cord at different stages of development in the North American opossum. A few serotoninergic axons are present in the lumbosacral cord at birth, 12 days after conception, and serotoninergic neurons are numerous in the brainstem where they are present in most, if not all, of the areas which contain them in the adult animals. A few neurons of the caudal raphe and adjacent reticular formation were labeled by lumbar injections of Fast blue on postnatal day 1, and by postnatal day 3, labeled neurons were numerous within all areas which provide serotoninergic projections to the lumbosacral cord in adult animals. By postnatal day 11, it was possible to combine Fast blue labeling with immunofluorescence to show that some of the labeled neurons were serotoninergic. By postnatal day 24, neurons which provide serotoninergic projections to the lumbosacral cord were especially numerous and some of them were found in areas which do not provide comparable projections in adult animals. In developing and adult animals, few, if any, neurons were labeled in the dorsal raphe or superior central nuclei. We have shown previously that serotoninergic axons do not innervate laminae I and II of the lumbosacral cord until approximately postnatal day 50, although they are present in the marginal zone at birth and have grown into laminae III-X by postnatal day 15. Since serotoninergic axons which project to laminae I and II originate within the raphe magnus and adjacent reticular formation, and those areas provide serotoninergic projections to the spinal cord well before postnatal day 50, it is possible that serotoninergic innervation of laminae I and II is provided by late growth of collaterals from axons that have been present in the marginal zone for some time.",
keywords = "Development, Opossum, Raphe nucleus, Reticular formation, Serotonin, Spinal cord",
author = "Martin, {G. F.} and G. Ghooray and Ho, {R. H.} and Pindzola, {R. R.} and Xiao-Ming Xu",
year = "1991",
month = "2",
day = "22",
doi = "10.1016/0165-3806(91)90006-5",
language = "English (US)",
volume = "58",
pages = "203--213",
journal = "Developmental Brain Research",
issn = "0165-3806",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - The origin of serotoninergic projections to the lumbosacral spinal cord at different stages of development in the North American opossum

AU - Martin, G. F.

AU - Ghooray, G.

AU - Ho, R. H.

AU - Pindzola, R. R.

AU - Xu, Xiao-Ming

PY - 1991/2/22

Y1 - 1991/2/22

N2 - We have employed immunohistochemistry and the retrograde transport of Fast blue to study the origin of serotoninergic projections to the lumbosacral spinal cord at different stages of development in the North American opossum. A few serotoninergic axons are present in the lumbosacral cord at birth, 12 days after conception, and serotoninergic neurons are numerous in the brainstem where they are present in most, if not all, of the areas which contain them in the adult animals. A few neurons of the caudal raphe and adjacent reticular formation were labeled by lumbar injections of Fast blue on postnatal day 1, and by postnatal day 3, labeled neurons were numerous within all areas which provide serotoninergic projections to the lumbosacral cord in adult animals. By postnatal day 11, it was possible to combine Fast blue labeling with immunofluorescence to show that some of the labeled neurons were serotoninergic. By postnatal day 24, neurons which provide serotoninergic projections to the lumbosacral cord were especially numerous and some of them were found in areas which do not provide comparable projections in adult animals. In developing and adult animals, few, if any, neurons were labeled in the dorsal raphe or superior central nuclei. We have shown previously that serotoninergic axons do not innervate laminae I and II of the lumbosacral cord until approximately postnatal day 50, although they are present in the marginal zone at birth and have grown into laminae III-X by postnatal day 15. Since serotoninergic axons which project to laminae I and II originate within the raphe magnus and adjacent reticular formation, and those areas provide serotoninergic projections to the spinal cord well before postnatal day 50, it is possible that serotoninergic innervation of laminae I and II is provided by late growth of collaterals from axons that have been present in the marginal zone for some time.

AB - We have employed immunohistochemistry and the retrograde transport of Fast blue to study the origin of serotoninergic projections to the lumbosacral spinal cord at different stages of development in the North American opossum. A few serotoninergic axons are present in the lumbosacral cord at birth, 12 days after conception, and serotoninergic neurons are numerous in the brainstem where they are present in most, if not all, of the areas which contain them in the adult animals. A few neurons of the caudal raphe and adjacent reticular formation were labeled by lumbar injections of Fast blue on postnatal day 1, and by postnatal day 3, labeled neurons were numerous within all areas which provide serotoninergic projections to the lumbosacral cord in adult animals. By postnatal day 11, it was possible to combine Fast blue labeling with immunofluorescence to show that some of the labeled neurons were serotoninergic. By postnatal day 24, neurons which provide serotoninergic projections to the lumbosacral cord were especially numerous and some of them were found in areas which do not provide comparable projections in adult animals. In developing and adult animals, few, if any, neurons were labeled in the dorsal raphe or superior central nuclei. We have shown previously that serotoninergic axons do not innervate laminae I and II of the lumbosacral cord until approximately postnatal day 50, although they are present in the marginal zone at birth and have grown into laminae III-X by postnatal day 15. Since serotoninergic axons which project to laminae I and II originate within the raphe magnus and adjacent reticular formation, and those areas provide serotoninergic projections to the spinal cord well before postnatal day 50, it is possible that serotoninergic innervation of laminae I and II is provided by late growth of collaterals from axons that have been present in the marginal zone for some time.

KW - Development

KW - Opossum

KW - Raphe nucleus

KW - Reticular formation

KW - Serotonin

KW - Spinal cord

UR - http://www.scopus.com/inward/record.url?scp=0026021888&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026021888&partnerID=8YFLogxK

U2 - 10.1016/0165-3806(91)90006-5

DO - 10.1016/0165-3806(91)90006-5

M3 - Article

C2 - 1851469

AN - SCOPUS:0026021888

VL - 58

SP - 203

EP - 213

JO - Developmental Brain Research

JF - Developmental Brain Research

SN - 0165-3806

IS - 2

ER -