The origin of the intrinsic glycoproteins of the rabbit vitreous body: An immunohistochemical and autoradiographic study

Antonio Haddad, Jorge C. de Almeida, Eduardo M. Laicine, Rose Fife, Georges Pelletier

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A cartilage matrix glycoprotein (CMGP), previously identified in human and bovine vitreous, now has been found in the vitreous body of rabbits aged 1-22 months by immunohistochemical techniques. Epithelial cells of the inner layer of the ciliary epithelium contain material that has immunologic crossreactivity with a specific antibody to CMGP. These cells also secrete glycoproteins, as determined by autoradiography after intravitreal injection of [3H]fucose. Approximately 14 bands, representing intrinsic glycoproteins containing fucose residues, can be identified in fluorograms of SDS-polyacrylamide gels of vitreous bodies from 6- and 22-month-old rabbits. Fluorograms of gels of samples of vitreous and ciliary bodies from several time points after intravitreal injection of [3H]fucose reveal at least seven comigrating protein bands and also demonstrate turnover of the labeled ciliary body glycoproteins. These results suggest that the inner layer of the ciliary epithelium is the source of the glycoproteins of the vitreous body and that these glycoproteins undergo turnover, probably throughout the entire life of the animals.

Original languageEnglish
Pages (from-to)555-561
Number of pages7
JournalExperimental Eye Research
Volume50
Issue number5
DOIs
StatePublished - 1990

Fingerprint

Vitreous Body
Glycoproteins
Fucose
Rabbits
Matrilin Proteins
Ciliary Body
Intravitreal Injections
Epithelium
Autoradiography
Gels
Epithelial Cells
Antibodies
Proteins

Keywords

  • cartilage matrix glycoprotein
  • ciliary body
  • glycoproteins
  • immuno-histochemistry
  • synthesis
  • vitreous

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

The origin of the intrinsic glycoproteins of the rabbit vitreous body : An immunohistochemical and autoradiographic study. / Haddad, Antonio; de Almeida, Jorge C.; Laicine, Eduardo M.; Fife, Rose; Pelletier, Georges.

In: Experimental Eye Research, Vol. 50, No. 5, 1990, p. 555-561.

Research output: Contribution to journalArticle

Haddad, Antonio ; de Almeida, Jorge C. ; Laicine, Eduardo M. ; Fife, Rose ; Pelletier, Georges. / The origin of the intrinsic glycoproteins of the rabbit vitreous body : An immunohistochemical and autoradiographic study. In: Experimental Eye Research. 1990 ; Vol. 50, No. 5. pp. 555-561.
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