The phospholipid and fatty acid composition of skeletal muscle cells during culture in the presence of vitamin D-3 metabolites

Teresita Bellido, Lila Drittanti, Ricardo Boland, Ana R. de Boland

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The phospholipid and fatty acid composition of primary cultures (24 h) of chick embryo skeletal muscle myoblasts treated for 4-24 h with physiological concentrations of 1,25-dihydroxyvitamin D-3 and 25-hydroxyvitamin D-3 were analyzed. 25-Hydroxyvitamin D-3 did not alter the relative amounts of individual muscle cell phospholipids whereas 1,25-dihydroxyvitamin D-3 significantly increased phosphatidylcholine content, mainly at the expense of a decrease in phosphatidylethanolamine concentration. The increase in phosphatidylcholine occurred at a faster rate during the first 8 h than in the subsequent 8-24 h treatment period. A similar time course in 1,25-dihydroxyvitamin D3-dependent changes in myoblast calcium uptake has been observed. In addition, this metabolite markedly increased (100%) the arachidonate content of myoblast phosphatidylcholine near the fusion stage of the cells (24 h of treatment). The levels of docosahexaenoate, a minor polyunsaturated fatty acid, in phosphatidylcholine and phosphatidylethanolamine were also substantially elevated by 1,25-dihydroxyvitamin D-3. No significant changes in fatty acid composition in response to 25-hydroxyvitamin D-3 were observed. Modifications in phospholipids and polyunsaturated fatty acids may play a role in the effects of 1,25-dihydroxyvitamin D-3 on muscle cell calcium transport and differentiation.

Original languageEnglish (US)
Pages (from-to)162-169
Number of pages8
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume922
Issue number2
DOIs
StatePublished - Nov 21 1987
Externally publishedYes

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Cholecalciferol
Metabolites
Phosphatidylcholines
Calcifediol
Cell culture
Muscle Cells
Muscle
Phospholipids
Skeletal Muscle
Fatty Acids
Cell Culture Techniques
Myoblasts
Chemical analysis
Unsaturated Fatty Acids
Cells
Skeletal Myoblasts
Calcium
Docosahexaenoic Acids
Cell Fusion
Calcitriol

Keywords

  • (Skeletal muscle cell)
  • Fatty acid composition
  • Phospholipid
  • Vitamin D-3 metabolite

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology
  • Medicine(all)

Cite this

The phospholipid and fatty acid composition of skeletal muscle cells during culture in the presence of vitamin D-3 metabolites. / Bellido, Teresita; Drittanti, Lila; Boland, Ricardo; de Boland, Ana R.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 922, No. 2, 21.11.1987, p. 162-169.

Research output: Contribution to journalArticle

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