The PIT-1 gene is regulated by distinct early and late pituitary-specific enhancers

Gabriel E. DiMattia, Simon J. Rhodes, Anna Krones, Catherine Carrière, Shawn O'Connell, Kristin Kalla, Carlos Arias, Paul Sawchenko, Michael G. Rosenfeld

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

The differentiation of three anterior pituitary cell types is regulated by the tissue-specific POU domain factor Pit-1, which is initially expressed on Embryonic Day 13.5-14 in mice. The Pit-1 gene remains continuously, highly expressed in the somatotrope, thyrotrope, and lactotrope cells of the adult. Using the Pit-1-defective Snell dwarf as a genetic background, we demonstrate that the Pit-1 gene utilizes distinct enhancers for initial gene activation and for subsequent autoregulation (required for maintenance of expression) and that Pit-1-dependent activation of the distal enhancer can be mediated in the absence of the early enhancer. These two distinct enhancers provide the basis for temporally specific regulation by discrete pituitary-specific factors, events likely to be prototypic for regulation of other classes of genes encoding transcription factors controlling terminal differentiation.

Original languageEnglish (US)
Pages (from-to)180-190
Number of pages11
JournalDevelopmental Biology
Volume182
Issue number1
DOIs
StatePublished - Feb 1 1997

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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