The PIT-1 gene is regulated by distinct early and late pituitary-specific enhancers

Gabriel E. DiMattia, Simon Rhodes, Anna Krones, Catherine Carrière, Shawn O'Connell, Kristin Kalla, Carlos Arias, Paul Sawchenko, Michael G. Rosenfeld

Research output: Contribution to journalArticle

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Abstract

The differentiation of three anterior pituitary cell types is regulated by the tissue-specific POU domain factor Pit-1, which is initially expressed on Embryonic Day 13.5-14 in mice. The Pit-1 gene remains continuously, highly expressed in the somatotrope, thyrotrope, and lactotrope cells of the adult. Using the Pit-1-defective Snell dwarf as a genetic background, we demonstrate that the Pit-1 gene utilizes distinct enhancers for initial gene activation and for subsequent autoregulation (required for maintenance of expression) and that Pit-1-dependent activation of the distal enhancer can be mediated in the absence of the early enhancer. These two distinct enhancers provide the basis for temporally specific regulation by discrete pituitary-specific factors, events likely to be prototypic for regulation of other classes of genes encoding transcription factors controlling terminal differentiation.

Original languageEnglish
Pages (from-to)180-190
Number of pages11
JournalDevelopmental Biology
Volume182
Issue number1
DOIs
StatePublished - Feb 1 1997

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POU Domain Factors
Genes
Transcriptional Activation
Homeostasis
Transcription Factors
Maintenance
Genetic Background

ASJC Scopus subject areas

  • Developmental Biology

Cite this

DiMattia, G. E., Rhodes, S., Krones, A., Carrière, C., O'Connell, S., Kalla, K., ... Rosenfeld, M. G. (1997). The PIT-1 gene is regulated by distinct early and late pituitary-specific enhancers. Developmental Biology, 182(1), 180-190. https://doi.org/10.1006/dbio.1996.8472

The PIT-1 gene is regulated by distinct early and late pituitary-specific enhancers. / DiMattia, Gabriel E.; Rhodes, Simon; Krones, Anna; Carrière, Catherine; O'Connell, Shawn; Kalla, Kristin; Arias, Carlos; Sawchenko, Paul; Rosenfeld, Michael G.

In: Developmental Biology, Vol. 182, No. 1, 01.02.1997, p. 180-190.

Research output: Contribution to journalArticle

DiMattia, GE, Rhodes, S, Krones, A, Carrière, C, O'Connell, S, Kalla, K, Arias, C, Sawchenko, P & Rosenfeld, MG 1997, 'The PIT-1 gene is regulated by distinct early and late pituitary-specific enhancers', Developmental Biology, vol. 182, no. 1, pp. 180-190. https://doi.org/10.1006/dbio.1996.8472
DiMattia, Gabriel E. ; Rhodes, Simon ; Krones, Anna ; Carrière, Catherine ; O'Connell, Shawn ; Kalla, Kristin ; Arias, Carlos ; Sawchenko, Paul ; Rosenfeld, Michael G. / The PIT-1 gene is regulated by distinct early and late pituitary-specific enhancers. In: Developmental Biology. 1997 ; Vol. 182, No. 1. pp. 180-190.
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