The pivotal role of VEGF on glomerular macrophage infiltration in advanced diabetic nephropathy

Waichi Sato, Tomoki Kosugi, Li Zhang, Carlos A. Roncal, Marcelo Heinig, Martha Campbell-Thompson, Yukio Yuzawa, Mark A. Atkinson, Maria B. Grant, Byron P. Croker, Takahiko Nakagawa

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A growing body of evidence implicates inflammation in the development of diabetic nephropathy. We recently reported that diabetic endothelial nitric oxide synthase knockout (eNOS KO) mice develop advanced glomerular lesions resembling human diabetic nephropathy. Vascular endothelial growth factor (VEGF) is a major factor in diabetic nephropathy, and is known to be chemotactic for macrophages. Herein, we examined the association of VEGF with macrophage infiltration in experimental diabetic nephropathy. Glomerular macrophage infiltration was markedly increased in diabetic eNOS KO mice compared to diabetic C57BL/6 mice, and correlated with glomerular injury, such as mesangiolysis, glomerular microaneurysm and nodular lesions of glomerular sclerosis. An elevation of podocyte VEGF expression correlated with infiltration of Flt-1-positive macrophage in injured glomeruli in diabetic eNOS KO mice, suggesting that VEGF could contribute to macrophage migration. Neither renal nNOS nor iNOS expression was altered in both C57BL/6 and eNOS KO mice. To determine if lack of NO could affect VEGF activation of macrophages, we examined if exogenous NO can block macrophage migration induced by VEGF in in vitro studies. Exogenous NO blocked macrophage migration and hypertrophy in response to VEGF. NO mediated these effects in part by downregulating Flt-1 expression on the macrophage. In summary, NO negatively regulates VEGF-induced macrophage migration by inhibiting Flt-1 expression. The VEGF-endothelial NO uncoupling pathway might partially explain how VEGF causes glomerular disease in diabetes.

Original languageEnglish (US)
Pages (from-to)949-961
Number of pages13
JournalLaboratory Investigation
Volume88
Issue number9
DOIs
StatePublished - Sep 2008
Externally publishedYes

Fingerprint

Diabetic Nephropathies
Vascular Endothelial Growth Factor A
Macrophages
Nitric Oxide Synthase Type III
Knockout Mice
Podocytes
Macrophage Activation
Sclerosis
Inbred C57BL Mouse
Hypertrophy
Down-Regulation
Inflammation
Kidney
Wounds and Injuries

Keywords

  • Diabetes
  • eNOS
  • Flt-1
  • NO

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Sato, W., Kosugi, T., Zhang, L., Roncal, C. A., Heinig, M., Campbell-Thompson, M., ... Nakagawa, T. (2008). The pivotal role of VEGF on glomerular macrophage infiltration in advanced diabetic nephropathy. Laboratory Investigation, 88(9), 949-961. https://doi.org/10.1038/labinvest.2008.60

The pivotal role of VEGF on glomerular macrophage infiltration in advanced diabetic nephropathy. / Sato, Waichi; Kosugi, Tomoki; Zhang, Li; Roncal, Carlos A.; Heinig, Marcelo; Campbell-Thompson, Martha; Yuzawa, Yukio; Atkinson, Mark A.; Grant, Maria B.; Croker, Byron P.; Nakagawa, Takahiko.

In: Laboratory Investigation, Vol. 88, No. 9, 09.2008, p. 949-961.

Research output: Contribution to journalArticle

Sato, W, Kosugi, T, Zhang, L, Roncal, CA, Heinig, M, Campbell-Thompson, M, Yuzawa, Y, Atkinson, MA, Grant, MB, Croker, BP & Nakagawa, T 2008, 'The pivotal role of VEGF on glomerular macrophage infiltration in advanced diabetic nephropathy', Laboratory Investigation, vol. 88, no. 9, pp. 949-961. https://doi.org/10.1038/labinvest.2008.60
Sato, Waichi ; Kosugi, Tomoki ; Zhang, Li ; Roncal, Carlos A. ; Heinig, Marcelo ; Campbell-Thompson, Martha ; Yuzawa, Yukio ; Atkinson, Mark A. ; Grant, Maria B. ; Croker, Byron P. ; Nakagawa, Takahiko. / The pivotal role of VEGF on glomerular macrophage infiltration in advanced diabetic nephropathy. In: Laboratory Investigation. 2008 ; Vol. 88, No. 9. pp. 949-961.
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