The posterior ventral tegmental area mediates alcohol-seeking behavior in alcohol-preferring rats

Sheketha R. Hauser, Zheng Ming Ding, Bruk Getachew, Jamie E. Toalston, Scott M. Oster, William J. McBride, Zachary Rodd

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The mesolimbic dopamine (DA) system is involved in the rewarding process of drugs of abuse and is activated during the anticipation of drug availability. However, the neurocircuitry that regulates ethanol (EtOH)-seeking has not been adequately investigated. The objectives of the present study were to determine 1) whether the posterior ventral tegmental area (p-VTA) mediates EtOH-seeking, 2) whether microinjections of EtOH into the p-VTA could stimulate EtOH-seeking, and (3) the involvement of p-VTA DA neurons in EtOH-seeking. Alcoholpreferring rats were trained to self-administer 15% EtOH and water. After 10 weeks, rats underwent extinction training, followed by 2 weeks in their home cages. During the home-cage period, rats were then bilaterally implanted with guide cannulae aimed at the p-VTA or anterior ventral tegmental area (a-VTA). EtOH-seeking was assessed by the Pavlovian spontaneous recovery model. Separate experiments examined the effects of: 1) microinjection of quinpirole into the p-VTA, 2) EtOH microinjected into the p-VTA, 3) coadministration of EtOH and quinpirole into the p-VTA, 4) microinjection of quinpirole into the a-VTA, and 5) microinjection of EtOH into the a-VTA. Quinpirole microinjected into the p-VTA reduced EtOH-seeking. Microinjections of EtOH into the p-VTA increased EtOH-seeking. Pretreatment with both quinpirole and EtOH into the p-VTA reduced EtOH-seeking. Microinjections of quinpirole or EtOH into the a-VTA did not alter EtOH-seeking. Overall, the results suggest that the p-VTA is a neuroanatomical substrate mediating alcohol-seeking behavior and that activation of local DA neurons is involved.

Original languageEnglish
Pages (from-to)857-865
Number of pages9
JournalJournal of Pharmacology and Experimental Therapeutics
Volume336
Issue number3
DOIs
StatePublished - Mar 2011

Fingerprint

Ventral Tegmental Area
Alcohols
Quinpirole
Microinjections
Dopaminergic Neurons
Street Drugs

ASJC Scopus subject areas

  • Pharmacology
  • Molecular Medicine

Cite this

The posterior ventral tegmental area mediates alcohol-seeking behavior in alcohol-preferring rats. / Hauser, Sheketha R.; Ding, Zheng Ming; Getachew, Bruk; Toalston, Jamie E.; Oster, Scott M.; McBride, William J.; Rodd, Zachary.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 336, No. 3, 03.2011, p. 857-865.

Research output: Contribution to journalArticle

Hauser, Sheketha R. ; Ding, Zheng Ming ; Getachew, Bruk ; Toalston, Jamie E. ; Oster, Scott M. ; McBride, William J. ; Rodd, Zachary. / The posterior ventral tegmental area mediates alcohol-seeking behavior in alcohol-preferring rats. In: Journal of Pharmacology and Experimental Therapeutics. 2011 ; Vol. 336, No. 3. pp. 857-865.
@article{85c3762d14b64fad9f1561a9d5b9429d,
title = "The posterior ventral tegmental area mediates alcohol-seeking behavior in alcohol-preferring rats",
abstract = "The mesolimbic dopamine (DA) system is involved in the rewarding process of drugs of abuse and is activated during the anticipation of drug availability. However, the neurocircuitry that regulates ethanol (EtOH)-seeking has not been adequately investigated. The objectives of the present study were to determine 1) whether the posterior ventral tegmental area (p-VTA) mediates EtOH-seeking, 2) whether microinjections of EtOH into the p-VTA could stimulate EtOH-seeking, and (3) the involvement of p-VTA DA neurons in EtOH-seeking. Alcoholpreferring rats were trained to self-administer 15{\%} EtOH and water. After 10 weeks, rats underwent extinction training, followed by 2 weeks in their home cages. During the home-cage period, rats were then bilaterally implanted with guide cannulae aimed at the p-VTA or anterior ventral tegmental area (a-VTA). EtOH-seeking was assessed by the Pavlovian spontaneous recovery model. Separate experiments examined the effects of: 1) microinjection of quinpirole into the p-VTA, 2) EtOH microinjected into the p-VTA, 3) coadministration of EtOH and quinpirole into the p-VTA, 4) microinjection of quinpirole into the a-VTA, and 5) microinjection of EtOH into the a-VTA. Quinpirole microinjected into the p-VTA reduced EtOH-seeking. Microinjections of EtOH into the p-VTA increased EtOH-seeking. Pretreatment with both quinpirole and EtOH into the p-VTA reduced EtOH-seeking. Microinjections of quinpirole or EtOH into the a-VTA did not alter EtOH-seeking. Overall, the results suggest that the p-VTA is a neuroanatomical substrate mediating alcohol-seeking behavior and that activation of local DA neurons is involved.",
author = "Hauser, {Sheketha R.} and Ding, {Zheng Ming} and Bruk Getachew and Toalston, {Jamie E.} and Oster, {Scott M.} and McBride, {William J.} and Zachary Rodd",
year = "2011",
month = "3",
doi = "10.1124/jpet.110.168260",
language = "English",
volume = "336",
pages = "857--865",
journal = "Journal of Pharmacology and Experimental Therapeutics",
issn = "0022-3565",
publisher = "American Society for Pharmacology and Experimental Therapeutics",
number = "3",

}

TY - JOUR

T1 - The posterior ventral tegmental area mediates alcohol-seeking behavior in alcohol-preferring rats

AU - Hauser, Sheketha R.

AU - Ding, Zheng Ming

AU - Getachew, Bruk

AU - Toalston, Jamie E.

AU - Oster, Scott M.

AU - McBride, William J.

AU - Rodd, Zachary

PY - 2011/3

Y1 - 2011/3

N2 - The mesolimbic dopamine (DA) system is involved in the rewarding process of drugs of abuse and is activated during the anticipation of drug availability. However, the neurocircuitry that regulates ethanol (EtOH)-seeking has not been adequately investigated. The objectives of the present study were to determine 1) whether the posterior ventral tegmental area (p-VTA) mediates EtOH-seeking, 2) whether microinjections of EtOH into the p-VTA could stimulate EtOH-seeking, and (3) the involvement of p-VTA DA neurons in EtOH-seeking. Alcoholpreferring rats were trained to self-administer 15% EtOH and water. After 10 weeks, rats underwent extinction training, followed by 2 weeks in their home cages. During the home-cage period, rats were then bilaterally implanted with guide cannulae aimed at the p-VTA or anterior ventral tegmental area (a-VTA). EtOH-seeking was assessed by the Pavlovian spontaneous recovery model. Separate experiments examined the effects of: 1) microinjection of quinpirole into the p-VTA, 2) EtOH microinjected into the p-VTA, 3) coadministration of EtOH and quinpirole into the p-VTA, 4) microinjection of quinpirole into the a-VTA, and 5) microinjection of EtOH into the a-VTA. Quinpirole microinjected into the p-VTA reduced EtOH-seeking. Microinjections of EtOH into the p-VTA increased EtOH-seeking. Pretreatment with both quinpirole and EtOH into the p-VTA reduced EtOH-seeking. Microinjections of quinpirole or EtOH into the a-VTA did not alter EtOH-seeking. Overall, the results suggest that the p-VTA is a neuroanatomical substrate mediating alcohol-seeking behavior and that activation of local DA neurons is involved.

AB - The mesolimbic dopamine (DA) system is involved in the rewarding process of drugs of abuse and is activated during the anticipation of drug availability. However, the neurocircuitry that regulates ethanol (EtOH)-seeking has not been adequately investigated. The objectives of the present study were to determine 1) whether the posterior ventral tegmental area (p-VTA) mediates EtOH-seeking, 2) whether microinjections of EtOH into the p-VTA could stimulate EtOH-seeking, and (3) the involvement of p-VTA DA neurons in EtOH-seeking. Alcoholpreferring rats were trained to self-administer 15% EtOH and water. After 10 weeks, rats underwent extinction training, followed by 2 weeks in their home cages. During the home-cage period, rats were then bilaterally implanted with guide cannulae aimed at the p-VTA or anterior ventral tegmental area (a-VTA). EtOH-seeking was assessed by the Pavlovian spontaneous recovery model. Separate experiments examined the effects of: 1) microinjection of quinpirole into the p-VTA, 2) EtOH microinjected into the p-VTA, 3) coadministration of EtOH and quinpirole into the p-VTA, 4) microinjection of quinpirole into the a-VTA, and 5) microinjection of EtOH into the a-VTA. Quinpirole microinjected into the p-VTA reduced EtOH-seeking. Microinjections of EtOH into the p-VTA increased EtOH-seeking. Pretreatment with both quinpirole and EtOH into the p-VTA reduced EtOH-seeking. Microinjections of quinpirole or EtOH into the a-VTA did not alter EtOH-seeking. Overall, the results suggest that the p-VTA is a neuroanatomical substrate mediating alcohol-seeking behavior and that activation of local DA neurons is involved.

UR - http://www.scopus.com/inward/record.url?scp=79951970589&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79951970589&partnerID=8YFLogxK

U2 - 10.1124/jpet.110.168260

DO - 10.1124/jpet.110.168260

M3 - Article

C2 - 21148248

AN - SCOPUS:79951970589

VL - 336

SP - 857

EP - 865

JO - Journal of Pharmacology and Experimental Therapeutics

JF - Journal of Pharmacology and Experimental Therapeutics

SN - 0022-3565

IS - 3

ER -