The potential roles of the conserved amino acids in human liver mitochondrial aldehyde dehydrogenase

Saifuddin Sheikh, Li Ni, Thomas Hurley, Henry Weiner

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Abstract

The sequence alignment of all known aldehyde dehydrogenases showed that only 23 residues were completely conserved (Hempel, J., Nicholas, H., and Lindahl, R. (1993) Protein Sci. 2, 1890-1900). Of these 14 were glycines and prolines. Site-directed mutagenesis showed that Cys302 was the essential nucleophile and that Glu268 was the general base necessary to activate Cys302 for both the dehydrogenase and esterase reaction. Here we report the mutational analysis of other conserved residues possessing, reactive side chains Arg84, Lys192, Thr384, Glu399, and Ser471, along with partially conserved Glu398 and Lys489, to determine their involvement in the catalytic process and correlate these finding with the known structure of mitochondrial ALDH (Steinmetz, C. G., Xie, P.-G., Weiner, H., and Hurley, T. D. (1997) Structure 5, 701-711). No residue was found to be absolutely essential, but all the mutations caused a decrease in the specific activity of the enzyme. None of the mutations affected the K(m) for aldehyde significantly, although k3, the rate constant calculated for aldehyde binding was decreased. The K(m) and dissociation constant (K(ia)) for NAD+ increased significantly for K192Q and S471A compared with the native enzyme. Mutations of only Lys192 and Glu399, both NAD+-ribose binding residues, led to a change in the rate-limiting step such that hydride transfer became rate-limiting, not deacylation. Esterase activity of all mutants decreased even though mutations affected different catalytic steps in the dehydrogenase reaction.

Original languageEnglish
Pages (from-to)18817-18822
Number of pages6
JournalJournal of Biological Chemistry
Volume272
Issue number30
DOIs
StatePublished - 1997

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Aldehyde Dehydrogenase
Esterases
Aldehydes
Liver
NAD
Oxidoreductases
Amino Acids
Mutagenesis
Mutation
Nucleophiles
Ribose
Enzymes
Proline
Hydrides
Glycine
Rate constants
Sequence Alignment
Site-Directed Mutagenesis
Proteins
Mitochondrial Aldehyde Dehydrogenase

ASJC Scopus subject areas

  • Biochemistry

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The potential roles of the conserved amino acids in human liver mitochondrial aldehyde dehydrogenase. / Sheikh, Saifuddin; Ni, Li; Hurley, Thomas; Weiner, Henry.

In: Journal of Biological Chemistry, Vol. 272, No. 30, 1997, p. 18817-18822.

Research output: Contribution to journalArticle

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