The presence of JAK2V617F mutation in the liver endothelial cells of patients with Budd-Chiari syndrome

Selcuk Sozer, Maria Isabel Fiel, Thomas Schiano, Mingjiang Xu, John Mascarenhas, Ronald Hoffman

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Patients with myeloproliferative disorders are at a high risk of developing thrombotic events. Several investigators have hypothesized that endothelial cell (EC) abnormalities might contribute to this prothrombotic state. Budd-Chiari syndrome (BCS) and portal vein thrombosis have been reported to be associated with JAK2V617F-positive hematopoiesis. We explored whether JAK2V617F was present in ECs in the vessels of polycythemia vera (PV) patients with BCS using laser capture microdissection followed by nested polymerase chain reaction or reverse-transcribed polymerase chain reaction. The ECs of the 2 BCS patients with PV were homozygous for the JAK2V617F and were shown to express transcripts characteristic of ECs but not hematopoietic cells. ECs of the other BCS patient with PV and 2 patients with hepatoportal sclerosis without PV contained exclusively wild-type JAK2. The presence of JAK2V617F in both ECs and hematopoietic cells belonging to BCS patients with PV indicate that ECs in PV are involved by the malignant process and that in a subpopulation of the patients the disease might originate from a common cell of origin for hematopoietic and ECs.

Original languageEnglish (US)
Pages (from-to)5246-5249
Number of pages4
JournalBlood
Volume113
Issue number21
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Budd-Chiari Syndrome
Polymerase chain reaction
Endothelial cells
Polycythemia Vera
Liver
Microdissection
Endothelial Cells
Mutation
Lasers
Laser Capture Microdissection
Myeloproliferative Disorders
Polymerase Chain Reaction
Hematopoiesis
Sclerosis
Portal Vein
Thrombosis
Research Personnel

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Sozer, S., Fiel, M. I., Schiano, T., Xu, M., Mascarenhas, J., & Hoffman, R. (2009). The presence of JAK2V617F mutation in the liver endothelial cells of patients with Budd-Chiari syndrome. Blood, 113(21), 5246-5249. https://doi.org/10.1182/blood-2008-11-191544

The presence of JAK2V617F mutation in the liver endothelial cells of patients with Budd-Chiari syndrome. / Sozer, Selcuk; Fiel, Maria Isabel; Schiano, Thomas; Xu, Mingjiang; Mascarenhas, John; Hoffman, Ronald.

In: Blood, Vol. 113, No. 21, 2009, p. 5246-5249.

Research output: Contribution to journalArticle

Sozer, S, Fiel, MI, Schiano, T, Xu, M, Mascarenhas, J & Hoffman, R 2009, 'The presence of JAK2V617F mutation in the liver endothelial cells of patients with Budd-Chiari syndrome', Blood, vol. 113, no. 21, pp. 5246-5249. https://doi.org/10.1182/blood-2008-11-191544
Sozer, Selcuk ; Fiel, Maria Isabel ; Schiano, Thomas ; Xu, Mingjiang ; Mascarenhas, John ; Hoffman, Ronald. / The presence of JAK2V617F mutation in the liver endothelial cells of patients with Budd-Chiari syndrome. In: Blood. 2009 ; Vol. 113, No. 21. pp. 5246-5249.
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