The primary structure of serum amyloid A protein in the rabbit

Comparison with serum amyloid A proteins in other species

Juris J. Liepnieks, Francis E. Dwulet, Merrill Benson, Barbara Kluve-Beckerman, Irving Kushner

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Rabbit serum amyloid A (SAA) protein was isolated from acute-phase serum by ultra-centrifugation, molecular seive chromatography, and ion-exchange chromatography. The complete amino acid sequence of the protein was established by sequence analysis of peptides derived from trypsin and Staphylococcus proteinase digestion of the protein. The molecule consisted of 104 amino acids and had an amino terminus that was blocked by pyrrolidonecarboxylic acid. Heterogeneity was not observed at any residue, which suggests that the material sequenced consisted of a single serum amyloid A species. The protein is highly homologous to serum amyloid A from humans and other animals, particularly in the middle portion of the molecule (positions 33 to 63), which suggests that this region may be important in its function. This highly conserved region may also contain the determinants for amyloid formation.

Original languageEnglish (US)
Pages (from-to)570-575
Number of pages6
JournalJournal of Laboratory and Clinical Medicine
Volume118
Issue number6
StatePublished - 1991

Fingerprint

Serum Amyloid A Protein
Chromatography
Rabbits
Pyrrolidonecarboxylic Acid
Amino Acids
Proteins
Molecules
Protein Sequence Analysis
Ion Exchange Chromatography
Centrifugation
Staphylococcus
Amyloid
Trypsin
Proteolysis
Amino Acid Sequence
Ion exchange
Animals
Peptide Hydrolases
Peptides
Serum

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

The primary structure of serum amyloid A protein in the rabbit : Comparison with serum amyloid A proteins in other species. / Liepnieks, Juris J.; Dwulet, Francis E.; Benson, Merrill; Kluve-Beckerman, Barbara; Kushner, Irving.

In: Journal of Laboratory and Clinical Medicine, Vol. 118, No. 6, 1991, p. 570-575.

Research output: Contribution to journalArticle

Liepnieks, Juris J. ; Dwulet, Francis E. ; Benson, Merrill ; Kluve-Beckerman, Barbara ; Kushner, Irving. / The primary structure of serum amyloid A protein in the rabbit : Comparison with serum amyloid A proteins in other species. In: Journal of Laboratory and Clinical Medicine. 1991 ; Vol. 118, No. 6. pp. 570-575.
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