The production of colony stimulating activity by monocyte enriched fractions from murine continuous bone marrow culture adherent layers

D. J. Oblon, H. Castro Malaspina, Hal Broxmeyer

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The production of colony stimulating activity (GM-CSA) within murine continuous bone marrow cultures was investigated by subjecting either the nonadherent or the adherent layer cell to separation by velocity sedimentation. The presence of GM-CSA in conditioned medium was defined by the support of granulocyte/macrophage colony formation in soft culture. Cell free conditioned medium from weekly feedings of intact continuous marrow cultures and medium conditioned by each fraction of velocity sedimentation separated, nonadherent cells did not contain assayable GM-CSA. However, medium conditioned by fractions of adherent layer cells with a modal sedimentation velocity of 8.8 mm/h (range 6.2-10.6 mm/h) contained GM-CSA. Cytochemical studies with Wrights-Giemsa and non-specific esterase stains in addition to immunofluoresent studies with anti-collagen III, andi-collagen IV and monoclonal anti-MAC I antibodies to define fibroblast, endothelial cells and monocytes, respectively, demonstrated that the cells within the GM-CSA producing fractions were enriched with monocytes/macrophages. Fibroblasts and a small proportion of endothelial cells were also present. GM-CSA is produced within the microenvironment (adherent layer) of murine continuous marrow cultures. Either adherent layer monocytes and/or a monocyte endothelial cell interaction account for the GM-CSA.

Original languageEnglish (US)
Pages (from-to)291-299
Number of pages9
JournalBritish Journal of Haematology
Volume54
Issue number2
StatePublished - 1983
Externally publishedYes

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Conditioned Culture Medium
Monocytes
Bone Marrow
Endothelial Cells
Collagen
Fibroblasts
Macrophages
Carboxylesterase
Cell Separation
Granulocytes
Cell Communication
Coloring Agents
Antibodies

ASJC Scopus subject areas

  • Hematology

Cite this

The production of colony stimulating activity by monocyte enriched fractions from murine continuous bone marrow culture adherent layers. / Oblon, D. J.; Castro Malaspina, H.; Broxmeyer, Hal.

In: British Journal of Haematology, Vol. 54, No. 2, 1983, p. 291-299.

Research output: Contribution to journalArticle

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