The proteome of normal pancreatic juice

Courtney J. Doyle, Kyle Yancey, Henry A. Pitt, Mu Wang, Kerry Bemis, Michele Yip-Schneider, Stuart Sherman, Keith D. Lillemoe, Michael D. Goggins, C. Schmidt

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

OBJECTIVES: The aims of this study were to characterize the proteome of normal pancreatic juice, to analyze the effect of secretin on the normal proteome, and to compare these results with published data from patients with pancreatic cancer. METHODS: Paired pancreatic fluid specimens (before and after intravenous secretin stimulation) were obtained during endoscopic pancreatography from 3 patients without significant pancreatic pathology. Proteins were identified and quantified by mass spectrometry-based protein quantification technology. The human RefSeq (NCBI) database was used to compare the data in samples from patients without pancreatic disease with published data from 3 patients with pancreatic cancer. RESULTS: A total of 285 proteins were identified in normal pancreatic juice. Ninety had sufficient amino acid sequences identified to characterize the protein with a high level of confidence. All 90 proteins were present before and after secretin administration but with altered relative concentrations, usually by 1 to 2 folds, after stimulation. Comparison with 170 published pancreatic cancer proteins yielded an overlap of only 42 proteins. CONCLUSIONS: Normal pancreatic juice contains multiple proteins related to many biological processes. Secretin alters the concentration but not the spectrum of these proteins. The pancreatic juice proteome of patients without pancreatic disease and that of patients with pancreatic cancer differ markedly.

Original languageEnglish
Pages (from-to)186-194
Number of pages9
JournalPancreas
Volume41
Issue number2
DOIs
StatePublished - Mar 2012

Fingerprint

Pancreatic Juice
Proteome
Secretin
Pancreatic Neoplasms
Proteins
Pancreatic Diseases
Biological Phenomena
Amino Acid Sequence
Mass Spectrometry
Databases
Pathology
Technology

Keywords

  • pancreatic adenocarcinoma
  • pancreatic fluid
  • proteome
  • secretin

ASJC Scopus subject areas

  • Hepatology
  • Internal Medicine
  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

The proteome of normal pancreatic juice. / Doyle, Courtney J.; Yancey, Kyle; Pitt, Henry A.; Wang, Mu; Bemis, Kerry; Yip-Schneider, Michele; Sherman, Stuart; Lillemoe, Keith D.; Goggins, Michael D.; Schmidt, C.

In: Pancreas, Vol. 41, No. 2, 03.2012, p. 186-194.

Research output: Contribution to journalArticle

Doyle, CJ, Yancey, K, Pitt, HA, Wang, M, Bemis, K, Yip-Schneider, M, Sherman, S, Lillemoe, KD, Goggins, MD & Schmidt, C 2012, 'The proteome of normal pancreatic juice', Pancreas, vol. 41, no. 2, pp. 186-194. https://doi.org/10.1097/MPA.0b013e31822862f6
Doyle, Courtney J. ; Yancey, Kyle ; Pitt, Henry A. ; Wang, Mu ; Bemis, Kerry ; Yip-Schneider, Michele ; Sherman, Stuart ; Lillemoe, Keith D. ; Goggins, Michael D. ; Schmidt, C. / The proteome of normal pancreatic juice. In: Pancreas. 2012 ; Vol. 41, No. 2. pp. 186-194.
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